Volume 4 Supplement 1

Antimicrobial Resistance and Infection Control: Abstracts from the 3rd International Conference on Prevention and Infection Control (ICPIC 2015)

Open Access

Determination of bacteria migration speed through urinary catheter systems in case of urostomy

  • FHH Brill1,
  • H Braunwarth2,
  • D Hegeholz2 and
  • W Droste3
Antimicrobial Resistance and Infection Control20154(Suppl 1):P219

DOI: 10.1186/2047-2994-4-S1-P219

Published: 16 June 2015

Introduction

Following a urostomy, the main aim from a hygiene perspective is to prevent bacteria from accumulating in the artificial drainage system (splint), e.g. as a result of contaminated urine. A return stop in the urostomy pouch keeps this risk to a minimum. In practice, however, splints are often pushed through the return stop to keep them more securely in place, which means that they may come into direct contact with the potentially contaminated urine.

Objectives

The Objective was to study the migration speed of clinically-relevant bacteria in catheter systems used after urostomy.

Methods

We carried out an in-vitro experiment in a commercially-available uriniferous system applied in a urostomy. This involved connecting two storage vessels: the first containing splints which had previously been rinsed once with artificial urine; and the second containing a bacterial suspension of the test bacteria (E. coli, P. aeruginosa and P. mirabilis), which had previously been soaked in artificial, sterile urine. The two storage vessels were inclubated at 36 °C for 24 to 72 hours. The splints were cut into segments of 5 cm after 24 hours, 48 hours and 72 hours. The colony-forming units (CFU) on the pieces were determined. Each experiment was carried out nine times before the average values and standard deviations were subsequently determined.

Results

After 24 hours the bacteria migrated into the splint, on average, as follows: E. coli 26.7 cm ± 20.6, S. aureus 27.2 cm ± 10.6 and P. mirabilis 12.8 cm ± 16.2. After 48 hours the bacteria migrated as follows: 35.0 cm ± 11.2 (E. coli), 51.7 cm ± 7.5 (S. aureus) and 41.7 cm ± 23.6 (P. mirabilis). The results after 72 hours were: 49.4 cm ± 14.5 (E. coli), 60 cm ± 16.0 (S. aureus) and 67.8 cm ± 3.6 (P. mirabilis).

Conclusion

The test bacteria grew relatively quickly through the catheter. It is likely that bacteria would grow through catheters with 80 cm length within a week at the latest. In this case, these is a direct infection risk for bladder and kidneys of the patient. These results should be taken into consideration during clinical use of the catheter systems in case urostomy.

Disclosure of interest

F. Brill Grant/Research support from: partially by Coloplast; Germany., H. Braunwarth Employee of: Coloplast, Germany., D. Hegeholz Employee of: Coloplast, Germany., W. Droste: None declared.

Authors’ Affiliations

(1)
Dr. Brill + Partner Gmbh Instiute for Hygiene and Microbiology
(2)
Coloplast GmbH
(3)
Private Consultant

Copyright

© Brill et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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