Volume 4 Supplement 1

Antimicrobial Resistance and Infection Control: Abstracts from the 3rd International Conference on Prevention and Infection Control (ICPIC 2015)

Open Access

Implementation of S. aureus surveillance for prevention of blood stream infections in hemodialysis patients

  • L Coucke1,
  • C Verfaillie1,
  • J Van Acker1,
  • M De Clippele2,
  • J Donck2 and
  • A-M Van den Abeele1
Antimicrobial Resistance and Infection Control20154(Suppl 1):P64

DOI: 10.1186/2047-2994-4-S1-P64

Published: 16 June 2015

Introduction

Surveillance for S. aureus (SA) nasal carriage followed by decolonization is a validated strategy to reduce infections and mortality in hemodialysis (HD) patients. This practice is not widespread in Belgian HD units. The current prevalence of methicillin sensitive SA (MSSA) and methicillin resistant SA (MRSA) carriers in our HD population is unknown.

Objectives

To evaluate the feasibility of routine surveillance and to assess SA prevalence, a point–prevalence survey measuring the colonization rate of HD patients was organised.

Methods

Screening of all HD patients was performed in January, June and November 2014. Home HD (n=1) and peritoneal dialysis (n=5) patients were excluded. Separate swabs (ESwab, Copan, Italy) were taken from anterior nares, wounds and the insertion site of the dialysis catheter. For MSSA detection, swabs were inoculated directly on chromogenic SA plates (SaSelectTM medium, Bio–Rad Laboratories, Belgium). MRSA was isolated on chromogenic MRSA plates (BDTM BBL CHROMagar MRSAII, Becton-Dickinson, Belgium) after overnight enrichment in tryptic soy broth of 100µL ESwab Amies medium. Suspected colonies were identified by gram stain and matrix–assisted laser desorption/ionization time–of–flight mass–spectrometry. MRSA confirmation was obtained by mecA gene and nuc gene polymerase chain reaction.

Results

154 patients (mean age 70 years, range 27-95 years) from 3 HD units were screened: 1 in-hospital unit (n=107), 2 outpatient centres (n=47). Mean dialysis time was 4.2 years. 14% of patients were in residential care. SA colonization rate was stable from January to November (26.3%). MRSA prevalence was 1.3% in January/June and 2.8% in November. Outpatient centres showed similar MSSA prevalence (33% and 28%) to the in-hospital unit (17%). 13 patients (9.2%) were persistent MSSA carriers, 8 of them carrying a central line (5.6%). None of the catheter insertion site swabs demonstrated SA.

Conclusion

Nasal MSSA prevalence in HD patients equals the general population prevalence (29%–32%), MRSA colonization rate (1.3%–2.8%) is low compared to the total in-hospital at-risk population (4%). Routine surveillance 3 times a year is achievable and should include MSSA and MRSA screening in all HD patients. New patients should be screened upon HD start.

Disclosure of interest

None declared.

Authors’ Affiliations

(1)
Laboratory of Clinical Microbiology, Laboratory Medicine, AZ Sint-Lucas Ghent
(2)
Nephrology Department, AZ Sint-Lucas Ghent

Copyright

© Coucke et al; licensee BioMed Central Ltd. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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