Multidrug resistant Enterobacteriaceae and extended spectrum β-lactamase producing Escherichia coli: a cross-sectional study in National Kidney Center, Nepal
© Yadav et al. 2015
Received: 10 August 2015
Accepted: 15 October 2015
Published: 26 October 2015
Emergence of antibacterial resistance and production of Extended spectrum β-lactamases (ESBLs) are responsible for the frequently observed empirical therapy failures. Most countries have experienced rapid dissemination of ESBLs producing Enterobacteriaceae isolates, particularly E. coli and Klebsiella pneumoniae. ESBLs are clinically significant and when detected, indicate the need for the use of appropriate antibacterial agents. But antibacterial choice is often complicated by multi-resistance.
This study was carried from June to November 2014 to study the multidrug resistant (MDR) Enterobacteriaceae and ESBL producing E. coli among urine isolates in hospital setting. Isolates from urine samples were primarily screened for possible ESBL production followed by phenotypic confirmation. Antibiotic susceptibility testing (AST) was done by Kirby Bauer disk diffusion method following Clinical and Laboratory Standard Institute (CLSI) guidelines.
Out of 450 urine samples processed, 141 significant growths were obtained including 95 Enterobacteriaceae isolates with 67 E. coli. Among Enterobacteriaceae, 92 (96.84 %) were recorded as MDR and 18 (26.87 %) E. coli were confirmed as ESBLs producers.
Using the phenotypic confirmatory test forwarded by the CLSI, relatively significant E. coli isolates tested were ESBL producers. Also high numbers of MDR organisms were isolated among Enterobacteriaceae. Isolates showed significant resistance to the commonly prescribed drugs. These findings suggest for further study in this field including the consequences of colonization with MDR and ESBL-producing bacteria both in the community and in the hospital setting.
KeywordsAntibiotic resistance E. coli Enterobacteriaceae ESBL Multidrug resistance Nepal Urine UTI
Urinary tract infection (UTI) is a common disease ailment among Nepalese population as well as one of the commonest nosocomial infection . Because of the evolving and continuing antibiotic resistance phenomenon, regular monitoring of resistance patterns is necessary to improve guidelines for empirical antibiotic therapy . Uropathogens have developed resistance to commonly prescribed antimicrobial agents; this severely limits the treatment options of an effective therapy. One of the important resistance mechanisms is production of enzymes destroying the drug β-lactam antibiotics. To date several types of β-lactamases have been characterized depending on the characteristic and hydrolytic activity. Extended spectrum β-lactamases (ESBLs) is one of the important groups of β-lactamases .
ESBLs are the enzymes that have the ability to hydrolyze and cause resistance to various types of newer β-lactam antibiotics, including the expanded-spectrum (or third generation) cephalosporins (eg. cefotaxime, ceftriaxone, ceftazidime) and monobactams (eg. aztreonam), but not the cephamycins (eg. cefoxitin and cefotetan) and carbapenems (eg. imipenem, meropenem and etrapenem) . These enzymes are sensitive to β-lactamase inhibitors (sulbactam, clavulanic acid, and tazobactam) .
A large number of outbreaks of the infections which are caused by ESBL producing organisms have been described in every continent of the globe . There is ample evidence to suggest the spread of ESBL infections is higher in resource poor countries . Major risk factors for colonization or infection with ESBL producing organisms are long term antibiotic exposure, prolonged intensive care unit (ICU) stay, nursing home residency, severe illness, residence in an institution with high rates of ceftazidime and other third generation cephalosporin use and instrumentation or catheterisation .
E. coli, that can produce ESBLs, has arisen and disseminated worldwide as an important cause of both nosocomial and community infections and nowadays represents a major threat. Early identification of potential ESBL carriers is the first step to withhold the dispersal of these microorganisms and to avoid possible complications . Since, ESBL production is usually plasmid mediated, it is possible for one specimen to contain both ESBL producing and non ESBL producing cells of the same species. This suggests that for optimal detection, several colonies must be tested from a primary culture plate . Adequate detection of ESBL-producing strains is crucial for appropriate choice of antimicrobial therapy and infection control measures .
MDR Enterobacteriaceae has been frequently reported from different parts of the world as an emergence of treatment problem. Antibiotics given empirically without proper antibiotic susceptibility testing are one of the major causes for the development of MDR. So, to ensure appropriate therapy, current knowledge of the organism that causes UTI and their antibiotic susceptibility is mandatory . The dissemination of ESBL-producing Enterobacteriaceae in the hospital setting is a problem with major therapeutic and epidemiological consequences . This study was aimed to investigate the current situation of ESBL-producing Escherichia coli among Enterobacteriaceae isolates and sensitivity pattern of isolates toward various chemotherapeutic agents.
This is a cross-sectional study conducted from June to November 2014 in National Kidney Center, Vanasthali, Kathmandu, Nepal. The study population included patients visiting the hospital suspected of UTIs and patients undergoing dialysis in the hospital.
Patients included in the study were given pre-labelled (date, time, identification code, age and sex), leak proof, sterile, screw-capped container to collect the mid-stream urine (MSU) sample. Urine samples from all age group were included in the study. Samples those held for more than two hours at room temperature and those without proper labelling were excluded from the study.
The collected urine specimens were processed in the Microbiology laboratory within 2 h of collection. Urine samples were streaked directly on MacConkey agar (MA) and Blood agar (BA) plates. These plates were incubated at 37 °C aerobically and after overnight incubation, they were checked for bacterial growth. The Gram negative isolates were identified by their colony morphology, Gram staining characteristics, catalase test, oxidase test, and other relevant biochemical tests as per standard laboratory methods of identification.
Antibiotic susceptibility testing of bacterial isolates was done by Kirby Bauer disk diffusion method following CLSI guidelines using Mueller Hilton Agar (MHA) . The discs were taken from HiMedia Laboratories (India). The followings are the concentrations of drugs used for disc diffusion testing: amikacin (30 μg), cefalexin (30 μg), cefixime (5 μg), cefotaxime (30 μg), ceftazidime (30 μg), ceftriaxone (30 μg), ciprofloxacin (5 μg), cotrimoxazole (23.75 μg sulfamethoxazole/1.25 μg trimethoprim), doxycycline (30 μg), imipenem (10 μg), nalidixic acid (30 μg), nitrofurantoin (300 μg), Norfloxacin (10 μg), and ofloxacin (5 μg). An isolate was considered as MDR if it was resistant to three or more drugs of different classes/groups of antibiotics.
All the E. coli isolates were subjected to the screening test for ESBL detection. Screening test for ESBL detection was done according to the CLSI guidelines . Isolates showing inhibition zone size of ≤ 22 mm with ceftazidime (30 μg), ≤ 25 mm with ceftriaxone (30 μg), and ≤ 27 mm with cefotaxime (30 μg) were interpreted as screening test positive for ESBL production.
For the confirmatory test for ESBL, two or three colonies of organisms were suspended in 0.5 ml of sterile broth and the turbidity matched to 0.5 McFarland. Using a sterile cotton swab the broth culture was uniformly swabbed on MHA. All the E. coli isolates which were resistant to at least ceftazidime, ceftriaxone and/or cefotaxime were subjected to the ESBL confirmatory test using ceftazidime (30 μg) and ceftazidime-clavulanic acid (30 μg + 10 μg) and the cefotaxime (30 μg) and cefotaxime-clavulanic acid (30 μg + 10 μg) combination disks. The tests were interpreted according to CLSI guidelines and a difference of 5 mm between zone of inhibition of a single disk and in combination with clavulanic acid (inhibitor) was confirmed to be produced by an ESBL positive isolate.
Age and gender wise distribution of isolates
21 – 40
41 – 60
Microbiological profile of urinary isolates
Antimicrobial resistance amongst Enterobacteriaceae (n = 95)
Resistance no. (%)
MDR trend in Enterobacteriaceae family
MDR strains (%)
Antimicrobial resistance among ESBL producing E. coli (n = 18)
Resistance no. (%)
This study was aimed to investigate ESBL-producing E. coli among Enterobacteriaceae isolates and sensitivity pattern of isolates toward various chemotherapeutic agents. Organisms producing ESBLs are clinically relevant and remain an important cause of failure of therapy with cephalosporins. ESBLs are primarily produced by the Enterobacteriaceae family, in particular K. pneumoniae and E. coli. Bacteria harbouring ESBLs may also acquire and most often exhibit additional resistances to other antimicrobial classes such as the quinolones, tetracyclines, cotrimoxazole, trimethoprim, and aminoglycosides, which further limits therapeutic options and thus pose a therapeutic dilemma .
Significant growth was obtained in 31.33 % urine culture samples. The majority of urine specimens showed no growth (68.67 %). The possible cause of low rate of growth positivity might be due to urine samples obtained from patients on antibiotics therapy, infection due to slow growing organisms or due to those organisms that were not able to grow on the routine media used [1, 14]. Number of female patients requesting for urine culture was higher than the male patients. Significant microbial growth was higher in case of female than in male. Urethral opening in females, short urethra and complicated physiology especially during pregnancy can be considered as reason . Female patients requesting for urine culture was higher, than the male patients, in age group of 21–40 years this may be because this age group consists sexually active women. Frequent or recent sexual activity is the most important risk factor for UTIs in young women. Nearly 80 % of all UTIs in premenopausal women occur within 24 h of intercourse. UTIs are very rare in celibate women. Certain types of contraceptives can also increase the risk of UTIs .
Numbers of gram negative organisms isolated were much higher than the gram positive. Similar predominance of gram negative organism in urine sample has been observed by other researchers too [17, 18]. Staphylococcus aureus, Coagulase-negative staphylococci (CoNS) and Enterococcus spp. were the gram positive organisms isolated. E. coli, Klebsiella spp., Proteus spp., Citrobacter spp., Pseudomonas spp. and Neisseria spp. were the gram negative organisms isolated. Among the gram negative organisms, Enterobacteriaceae were most frequent, 95 out of 100 gram negative organisms. Members of Enterobacteriaceae are more likely to cause UTIs than other organisms. In various studies predominant organisms isolated in UTI cases is Enterobacteriaceae [17, 19, 20].
Antibiotic susceptibility pattern shown by the Enterobacteriaceae isolates were variable. Imipenem was the most effective antibiotic as 92.63 % of isolates were susceptible, followed by amikacin (82.11 %). Isolates were comparatively less susceptible to cephalosporins than other antibiotics. Resistance to β-lactams in Enterobacteriaceae is mainly due to the production of β-lactamases, which may be encoded either chromosomally or on plasmids . Out of 14 antibiotics used, 11 were found effective to only less than half of the isolates. Majority (96.84 %) of Enterobacteriaceae isolates were found to be MDR. Thakur et al.  has observed 64.04 % MDR Enterobacteriaceae and 73.68 % MDR E. coli isolates. The widespread use of antibiotics could be associated with the selection of antibiotic resistance mechanisms in pathogenic and non pathogenic isolates of E. coli . MDR isolates were more in females than in males and common in age group ≥ 61 years.
Over the past few years, the prevalence of ESBL producing strains among clinical isolates varies greatly with different geographic regions and rapidly changing over time . In this study, phenotypically 18 (26.87 %) isolates were confirmed as ESBL producers E. coli isolates. ESBL positive E. coli was distributed equally among male and female. Highest number of ESBL producers E. coli was obtained from the patients of age above 60 years. Other studies have also shown that ESBL isolates are encountered more frequently in the elderly, according to Roshan et al. , Shah et al.  and Rajan and Prabavathy  majority of isolates were from patients between 40 to 70 years, 50 to 60 years and 51 to 70 years respectively. The ESBLs-producing E. coli were most frequent in older age group in this study; it can be due to the reason that older patients are immunocompromised and more prone to infections by resistant organisms . Nosocomial infections caused by ESBL producing pathogens are associated with risk factors such as elderly age, prolonged hospitalization, previous antibiotic use, and presence of invasive devices .
All ESBL positive E. coli strains were resistant to cefotaxime, ceftazidime and ceftriaxone. This outcome is in agreement with the study done by Islam et al. . Similarly all E. coli isolates were resistant to cefotaxime and ceftriaxone in a study by Sompolinsky et al.  and to ceftazidime and ceftriaxone in a study by Chander and Shrestha . High percentage of resistance to cefotaxime (99.2 %), ceftazidime (99.2 %) and ceftriaxone (99.5 %) was observed by Wani et al. . ESBL positive isolates also showed high degree of resistance to other antibiotics like cefalexin, norfloxacin, cefixime, nalidixic acid, ciprofloxacin and ofloxacin. Aminoglycosides have good activity against clinically important gram negative bacilli . Aminoglycosides are very important group of antibiotics with activity against many gram-negative rods and the most common mechanism of aminoglycoside resistance is enzymatic modification of antibiotic molecule. All ESBL positive isolates were sensitive to the amikacin (100 %) followed by imipenem (94.44 %) and nitrofurantoin (72.22 %). Antimicrobial resistance surveillance done Nepal Public Health Laboratory (NPHL) found that ESBL E. coli were susceptible to imipenem (98.5 %), amikacin (96.1 %) followed by nitrofurantoin (89.2 %) and chloramphenicol (90.8 %) . Amikacin and nitrofurantoin can therefore be used effectively against ESBL producing isolates but these antibiotics have many limitations. High percentage of isolates were susceptible to the carbapenem. The study done by Kader and Angamuthu  revealed more than 89 % of the ESBL producers were susceptible to imipenem and meropenem, whereas Mekki et al.  found 100 % isolates sensitive to the carbapenems. The production of β-lactamase may be of chromosomal or plasmid origin [4, 36]. Plasmid mediated production is often acquired by transfer of genetic information from one organism to another. Such transferable plasmid also codes for resistant determinants to antimicrobial agents other than β-lactams . Hence multidrug resistance is expected to be more common in ESBL producing organisms.
The production of ESBL pathogens like E. coli has an important clinical importance. It has been well recognized that poor outcome occurs when patients with serious infections due to ESBL-producing organisms are treated with antibiotics like cephalosporins and penicillins to which the organisms are resistant. The mortality rate in such patients is significantly higher than in patients treated with antibiotics to which the organism is susceptible. All patients with antibiotics failure either die or have continued sign of infections, which necessitates change in antibiotic . Microbiology laboratories can play an important role in detecting and promptly reporting the isolation of ESBL-positive bacteria, since drug susceptibility data are important for the clinical management of patients infected by these organisms . Clinicians, whose laboratories do not perform tests for detection of ESBLs, and report ESBL producers as resistant to cephalosporins, risk poor outcome for their patients infected with ESBL producing organisms. The detection of ESBLs in any clinical isolate has great potential significance from the point of view of infection control .
This study also has some limitations; the study was carried out in a hospital. The picture of the study does not necessarily reveal the picture of the whole country, therefore systematic prospective surveillance should be carried covering wide geographical region in order to obtain information on seasonal, geographical and ethnic variation of pathogens and their antibiotic susceptibility profile. Moreover, characterization of ESBL strains should be performed genotypically, so that the information can be used in fighting with increasing resistance to antimicrobials.
The present study looked at the ESBL production in E. coli isolates in National Kidney Center in Kathmandu, Nepal. Using the phenotypic confirmatory test forwarded by the CLSI, relatively significant E. coli isolates tested were ESBL producers. Multidrug resistance to the antibiotics tested was also seen more among ESBL producer than the non-producers. In this study Enterobacteriaceae isolates showed significant resistance to the commonly prescribed drugs. In the present study 96.84 % Enterobacteriaceae were found to be multidrug resistant and 26.87 % E. coli were ESBL producers. These findings suggest for further study in this field including the consequences of colonization with multidrug resistant and ESBL-producing bacteria both in the community and in the hospital setting. These findings also suggest incorporating early detection mechanism of ESBL by clinical setting so that appropriate antibiotics can be used and this will also help in controlling increasing multidrug resistance due empirical therapy. With the spread of ESBL producing strains in hospitals all over the world, it is necessary to know the prevalence of ESBL positive strains in a hospital so as to formulate a policy of empirical therapy in high risk units where infections due to resistance organisms is higher.
Antibiotic susceptibility testing
Clinical and Laboratory Standard Institute
Extended spectrum β-lactamase
Intensive care unit
Mueller Hilton agar
Nepal Public Health Laboratory
Urinary tract infection
The authors would like to acknowledge Kantipur College of Medical Science and National Kidney Center.
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- Kattel HP, Acharya J, Mishra SK, Rijal BP, Pokhrel BM. Bacteriology of urinary tract infection among patients attending Tribhuvan University Teaching Hospital, Kathmandu, Nepal. J Nepal Assoc Med Lab Sci. 2008;9:25–9.Google Scholar
- Nachimuthu R, Sumathi CS, Balasubramanian V, Palaniappan KR, Kannan VR. Urinary tract infection and antimicrobial susceptibility pattern of extended spectrum of beta lactamase producing clinical isolates. Adv Biol Res. 2008;2:78–82.Google Scholar
- Thirapanmethee K. Extended spectrum β-lactamases: critical tools of bacterial resistance. Mahidol Univ J Pharm Sci. 2012;39:1–8.Google Scholar
- Bradford PA. Extended-Spectrum β-Lactamases in the 21st Century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev. 2001;14:933–51.PubMed CentralView ArticlePubMedGoogle Scholar
- Mukherjee M, Basu S, Mukherjee SK, Majumder M. Multidrug-resistance and extended spectrum beta-lactamase production in uropathogenic E. coli which were isolated from hospitalized patients in Kolkata, India. J Clin Diagn Res. 2013;7:449–53.PubMed CentralPubMedGoogle Scholar
- Chander A, Shrestha CD. Prevalence of extended spectrum beta lactamase producing Escherichia coli and Klebsiella pneumoniae urinary isolates in a tertiary care hospital in Kathmandu, Nepal. BMC Res Notes. 2013;6:487–92.PubMed CentralView ArticlePubMedGoogle Scholar
- Chaudhary U, Aggarwal R. Extended spectrum β-lactamases (ESBL) - An emerging threat to clinical therapeutics. Indian J Med Microbiol. 2004;22:75–80.PubMedGoogle Scholar
- Sahuquillo-Arce JM, Perpinan H, Armero C, Lopez-Quilez A, Selva M, Gonzalez F. Bayesian Approach to Urinary ESBL-Producing Escherichia coli. J Pharmacovigilance. 2014;2:133.Google Scholar
- Cohen Stuart J, Dierikx C, Al Naiemi N, Karczmarek A, Van Hoek AHAM, Vos P, et al. Rapid detection of TEM, SHV and CTX-M extended-spectrum β-lactamases in Enterobacteriaceae using ligation-mediated amplification with microarray analysis. J Antimicrob Chemother. 2010;65:1377–81.View ArticlePubMedGoogle Scholar
- Tambekar DH, Dhanorkar DV, Gulhane SR, Khandelwal VK, Dudhane MN. Antibacterial susceptibility of some urinary tract pathogens to commonly used antibiotics. Afr J Biotechnol. 2006;5:1562–65.Google Scholar
- Mena A, Plasencia V, García L, Hidalgo O, Ayestarán JI, Alberti S, et al. Characterization of a Large Outbreak by CTX-M-1-Producing Klebsiella pneumoniae and Mechanisms Leading to In Vivo Carbapenem Resistance Development. J Clin Microbiol. 2006;44:2831.PubMed CentralView ArticlePubMedGoogle Scholar
- Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing. USA: CLSI: M100-S25; 2015. Wayne, PA.Google Scholar
- Maina D, Makau P, Nyerere A, Revathi G. Antimicrobial resistance patterns in extended-spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae isolates in a private tertiary hospital, Kenya. Microb Discov. 2013;1:5.View ArticleGoogle Scholar
- Sharma AR, Bhatta DR, Shrestha J, Banjara MR. Antimicrobial Susceptibility Pattern of Escherichia coli isolated from Urinary Tract Infected Patients Attending Bir Hospital. Nepal J Sci Technol. 2013;14:177–84.View ArticleGoogle Scholar
- Moyo SJ, Aboud S, Kasubi M, Lyamuya EF, Maselle SY. Antimicrobial resistance among producers and non-producers of extended spectrum beta-lactamases in urinary isolates at a tertiary Hospital in Tanzania. BMC Res Notes. 2010;3:348–52.PubMed CentralView ArticlePubMedGoogle Scholar
- Simon H. Urinary Tract Infection. 2015. http://pennstatehershey.adam.com/content.aspx?productId=10&pid=10&gid=000036. Accessed 05 Sep 2015.Google Scholar
- Dhakal S, Manandhar S, Shrestha B, Dhakal R, Pudasaini M. Extended spectrum β-lactamase producing multidrug resistant urinary isolates from children visiting Kathmandu Model Hospital. Nepal Med Coll J. 2012;14:136–41.PubMedGoogle Scholar
- Ahmed OB, Omar AO, Asghar AH, Elhassan MM. Increasing prevalence of ESBL-producing Enterobacteriaceae in Sudan community patients with UTIs. Egypt Acad J Biolog Sci. 2013;5:17–24.Google Scholar
- Acharya A, Gautam R, Subedee L. Uropathogens and their antimicrobial susceptibility pattern in Bharatpur, Nepal. Nepal Med Coll J. 2011;13:30–3.PubMedGoogle Scholar
- Khawcharoenporn T, Vasoo S, Singh K. Urinary Tract Infections due to Multidrug-Resistant Enterobacteriaceae: Prevalence and Risk Factors in a Chicago Emergency Department. Hindawi Publishing Corporation Emergency Medicine International. 2013; doi:10.1155/2013/258517
- Thakur S, Pokhrel N, Sharma N. Prevalence of Multidrug Resistant Enterobacteriaceae and Extended Spectrum β Lactamase Producing Escherichia Coli in Urinary Tract Infection. RJPBCS. 2013;4:1615–24.Google Scholar
- Sorum H, Sunde M. Resistance to antibiotics in the normal flora of animals. Vet Res. 2001;32:227–41.View ArticlePubMedGoogle Scholar
- Kandeel A. Prevalence and risk factors of extended-spectrum β-lactamses producing Enterobacteriaceae in a general hospital in Saudi Arabia. JMID. 2014;4:50–4.View ArticleGoogle Scholar
- Roshan M, Ikram A, Mirza IA, Malik N, Abbasi SA, Alizai SA. Susceptibility Pattern of Extended Spectrum ß-Lactamase Producing Isolates in Various Clinical Specimens. J Coll Physicians Surg Pak. 2011;21:342–6.PubMedGoogle Scholar
- Shah AA, Hasan F, Ahmed S, Hameed A. Extended-spectrum beta-lactamases in Enterobacteriaceae: related to age and gender. New Microbiol. 2002;25:363–6.PubMedGoogle Scholar
- Rajan S, Prabavathy J. Antibiotic Sensitivity and Phenotypic Detection of ESBL producing E. coli Strains Causing Urinary Tract Infection In a Community Hospital, Chennai, Tamil Nadu, India. WebmedCentral. Pharm Sci. 2012;3, WMC003840.Google Scholar
- Mumtaz S, Ahmad M, Aftab I, Akhtar N, ul Hassan M, Hamid A. Extended spectrum β-lactamases in enteric gram-negative bacilli: related to age and gender. JAMC. 2007;19:107–11.PubMedGoogle Scholar
- Khanfar HS, Bindayna KM, Senok AC, Botta GA. Extended spectrum beta-lactamases (ESBL) in Escherichia coli and Klebsiella pneumoniae: trends in the hospital and community settings. J Infect Dev Ctries. 2009;3:295–9.PubMedGoogle Scholar
- Islam MS, Yusuf MA, Islam MB, Jahan WA. Frequency of ESBL in Surgical Site Infection at a Tertiary Care Hospital. J Curr Adv Med Res. 2014;1:25–9.Google Scholar
- Sompolinsky D, Nitzan Y, Tetry S, Wolk M, Vulikh I, Kerrn MB, et al. Integron-mediated ESBL resistance in rare serotypes of Escherichia coli causing infections in an elderly population of Israel. J Antimicrob Chemother. 2005;55:119–22.View ArticlePubMedGoogle Scholar
- Wani KA, Thakur MA, Fayaz AS, Fomdia B, Gulnaz B, Maroof P. Extended Spectrum B-Lactamase Mediated Resistance in Escherichia Coli in a Tertiary Care Hospital. Int J Health Sci (Qassim). 2009;3:155–63.Google Scholar
- Gonzalez US, Spencer JP. Aminoglycosides: a practical review. Am Fam Physician. 1998;58:1811–20.PubMedGoogle Scholar
- Nepal Public Health Laboratory (NPHL). Dissemination of important findings of Anti Microbial Resistance Surveillance programme. http://www.nphl.gov.np/index.php?obj=content&id=161. Accessed 22 June 2014.
- Kader AA, Angamuthu K. Extended-spectrum beta-lactamases in urinary isolates of Escherichia coli, Klebsiella pneumoniae and other gram-negative bacteria in a hospital in Eastern Province, Saudi Arabia. Saudi Med J. 2005;26:956–9.PubMedGoogle Scholar
- Mekki AH, Hassan AN, Elsayed DEM. Extended spectrum beta lactamase among multi drug resistant E. coli and Klebsiella species causing urinary tract infections in Khartoum. J Bact Res. 2010;2:18–21.Google Scholar
- Smet A, Martel A, Persoons D, Dewulf J, Heyndrickx M, Herman L, et al. Broad-spectrum β-lactamases among Enterobacteriaceae of animal origin: molecular aspects, mobility and impact on public health. FEMS Microbiol Rev. 2010;34:295–316.View ArticlePubMedGoogle Scholar
- Paterson DL. Resistance in gram-negative bacteria: Enterobacteriaceae. Am J Med. 2006;119:S20–8.View ArticlePubMedGoogle Scholar
- Paterson DL, Ko WC, Gottberg AV, Casellas JM, Mulazimoglu L, Klugman KP, et al. Outcome of cephalosporin treatment for serious infections due to apparently susceptible organisms producing extended spectrum β-lactamases: implications for the clinical microbiology laboratory. J Clin Microbiol. 2001;39:2206–12.PubMed CentralView ArticlePubMedGoogle Scholar
- Shah AA, Hasan F, Ahmed S, Hameed A. Prevalence of extended spectrum β-lactamases in nosocomial and outpatients (ambulatory). Pak J Med Sci. 2003;19:187–91.Google Scholar