Genetic markers associated with resistance to beta-lactam and quinolone antimicrobials in non-typhoidal Salmonella isolates from humans and animals in central Ethiopia

Table 1 List of primers used for detection and characterization of beta-lactamases

Gene/target	Primer	Sequence 5’-3’	Amplicon size	AT °C	Ref	Remark
BLA_Tem Gene	TEM-F1	ATGAGTATTCAACATTTCCG	862-bp	55	[34]
	TEM-R1	GACAGTTACCAATGCTTAATCA	862-bp	55	[34]
	blaTEM-F2	TAA CCA TGAGTGATAACACT			[34]	sequencing
	blaTEM-R2	CCGATCGTT GTCAGAAGTAA			[34]	sequencing
BLA _SHV gene	Bla SHV-F1	CTTTACTCGCCTTTATCG	827-bp	56	[34]
	Bla SHV-R1	TCCCGC AGATAAATCACCA	827-bp	56	[34]
	blaSHV-F2	ACTGCCTTTTTG CGCCAGAT			[34]	sequencing
	blaSHV-R2	CAGTTCCGTTTCCCAGCGGT			[34]	sequencing
Bla OXA-1	OXA-1-F	ATGAAAAACACAATACATATCAAC	755-bp	48	[13]
Bla OXA-1	OXA-1-R	TTTCCTGTAAGTGCGGACAC	755-bp	48	[13]
Bla OXA −4	OXA-4-F	TCAACAGATATCTCTACTGGT	216 bp	54	[13]
Bla OXA −4	OXA-4-R	TTTATCCCATTTGAATATG	216 bp	54	[13]
Bla OXA-10	OXa 10-F	TCAACAAATCGCCAGAGAAG	277 bp	57	[13]
Bla OXA-10	Oxa-10-R	TCCCACACCAGAAAAACCA	277 bp	57	[13]
bla PER	Per1-F	AATTTGGGCTTAGGGCAGAA	925 bp	55	[50]
bla PER	Per1-R	ATGAATGTCATTATAAAAGC	925 bp	55	[50]
blaPSE	blaPSE-F	TGCTTCGCAACTATGACTAC			[42]
blaPSE	blaPSE-R	AGCCTGTGTTTGAGCTAGAT			[42]
blaCYM	blaCMY2-F	TGGCCGTTGCCGTTATCTAC	868	57	[9]
blaCYM	blaCMY2-R	CCCGTTTATGCACCCATGA	868	57	[9]
CTX-M group I	CTXM1-F3	GACGATGTCACTGGCTGAGC	499	55	[38]
CTX-M group I	CTXM1-R2	AGCCGCCGACGCTAATACA	499	55	[38]
CTX-M group II	TOHO1-2 F	GCGACCTGGTTAACTACAATCC	351	55	[38]
CTX-M group II	TOHO1-1R	CGGTAGTATTGCCCTTAAGCC	351	55	[38]
CTX-M group III	CTXM825F	CGCTTT GCCATGTGCAGCACC	307	55	[38]
CTX-M group III	CTXM825R	GCT CAGTACGATCGAGCC	307	55	[38]
CTX-M group IV	CTXM914F	GCTGGAGAAAAGCAGCGGAG	474	62	[38]
CTX-M group IV	CTXM914R	GTAAGCTGACGCAACGTCTG

ISSN: 2047-2994