Genetic markers associated with resistance to beta-lactam and quinolone antimicrobials in non-typhoidal Salmonella isolates from humans and animals in central Ethiopia

Table 2 List of primers used for detection of quinolone resistance mechanism

Gene	Primer name	Primer sequence (5′ to 3′)	Product size	AT in °C	References
gyrA	GyrAFP	AAATCTGCCCGTGTCGTTGGT	344 bp	58	[16]
gyrA	GyrARP	GCCATACCTACTGCGATACC	344 bp	58	[16]
gyrB	GyrB FP	GAATACCTGCTGGAAAACCCAT	446 bp	57	[16]
gyrB	GyrB RP	CGGATGTGCGAGCCGTCGACGTCCGC	446 bp	57	[16]
parC	ParC FP	AAGCCGGTACAGCGCCGCATC	395 bp	57	[16]
parC	ParC RP	GTGGTGCCGTTCAGCAGG	395 bp	57	[16]
ParE	ParE FP	TCTCTTCCGATGAAGTGCTG	240 bp	55	[12]
ParE	ParE RP	ATACGGTATAGCGGCGGTAG	240 bp	55	[12]
qnrA	qnrA FP	ATTTCTCACGCCAGGATTTG	516 bp	53	[43]
qnrA	qnrA RP	GATCGGCAAAGGTTAGGTCA	516 bp	53	[43]
qnrB	qnrB FP	GATCGTGAAAGCCAGAAAGG	469 bp	53	[43]
qnrB	qnrB RP	ACGATGCCTGGTAGTTGTCC	469 bp	53	[43]
aac(6’)-Ib	aac(6’)-Ib FP	TTGCGATGCTCTATGAGTGGCTA	482-bp	55	[36]
aac(6’)-Ib	aac(6’)-Ib-RP	CTCGAATGCCTGGCGTGTTT	482-bp	55	[36]
	aac(6’)-Ib-cr-seq	CGTCACTCCATACATTGCAA (for sequencing of aac(6’)-Ib-cr
qepA	QepA FP	CGTGTTGCTGGAGTTCTTC	403 bp	59	[7]
qepA	QepA RP	CTGCAGGTACTGCGTCATG	403 bp	59	[7]
QnrD	QnrD FP	CGAGATCAATTTACGGGGAATA	565 bp	53	[8]
QnrD	QnrD RP	AACAAGCTGAAGCGCCTG	565 bp	53	[8]
QnrS	QnrS FP	ACGACATTCGTCAACTGCAA	417 bp	53	[43]
QnrS	QnrS RP	TAAATTGGCACCCTGTAGGC	417 bp	53	[43]

ISSN: 2047-2994