Co-existence of bla OXA-23 and bla NDM-1 genes of Acinetobacter baumannii isolated from Nepal: antimicrobial resistance and clinical significance

Table 1 List of primer for detection of genes used in this study

Target genes	Primer name	Sequence 5’-3’	Size/ Annealing temp.	References
16S rRNA	16S rRNA-F	AGAGTTTGATCCTGGCTCAG	1500/58	[15]
	16S rRNA-R	ACGGCTACCTTGTTACGACTT
bla _OXA-23	bla _OXA-23-F	GATCGGATTGGAGAACCAGA	501/52	[16]
	bla _OXA-23-R	ATTTCTGACCGCATTTCCAT
bla _OXA-51	bla _OXA-51-F	TAATGCTTTGATCGGCCTTG	353/52	[16]
	bla _OXA-51-R	TGGATTGCACTTCATCTTGG
bla _OXA-24	bla _OXA-24-F	GGTTAGTTGGCCCCCTTAAA	246/52	[16]
	bla _OXA-24-R	AGTTGAGCGAAAAGGGGATT
bla _OXA-58	bla _OXA-58-F	AAGTATTGGGGCTTGTGCTG	599/52	[16]
	bla _OXA-58-R	CCCCTCTGCGCTCTACATAC
bla _IMP	bla _IMP –F	GGAATAGAGTGGCTTAAYTCTC	232/52	[20]
	bla _IMP –R	GGTTTAAYAAAACAACCACC
bla _VIM	bla _VIM –F	GATGGTGTTTGGTCGCATA	390/52	[20]
	bla _VIM –R	CGAATGCGCAGCACCAG
bla _NDM	bla _NDM–F	GGTTTGGCGATCTGGTTTTC	621/52	[21]
	bla _NDM–R	CGGAATGGCTCATCACGATC
bla _ADC	bla _ADC-F	TAAACACCACATATGTTCCG	663/56	[19]
	bla _ADC-F	ACTTACTTCAACTCGCGACG
aphA6	aphA6-F	ATGGAATTGCCCAATATTATTC	736/55	[14]
	aphA6-R	TCAATTCAATTCATCAAGTTTTA
ISAba1	ISAba1-F	CATTGGCATTAAACTGAGGAGAAA	451/52	[22]
	ISAba1-R	TTGGAAATGGGGAAAACGAA
ISAba125	ISA125-F	TGTTGAAGCGATCCGTTGTT	755/57	This study
	ISA125-R	GTGCGACAGTTTCAAAAGCCA
Rep-PCR	ERIC-2	AAGTAAGTGACTGGGGTGAGCG	variable length/45	[24]

ISSN: 2047-2994