Table 1 Primers used for PCR amplification and sequencing
From: Characterization of the novel In1059 harbouring VIM gene cassette
Primer | Sequence (5′-3′) | Length (bp) | Reference | |
|---|---|---|---|---|
5′-CS | F | GGCATCCAAGCAGCAAGC | Variable | [40] |
3′-CS | R | AAGCAGACTTGACCTGAT | ||
Int1 | F | AGCACCTTGCCGTAGAAGAACAG | 3500 | This study |
R | GTCATAATCGGTTATGGCATCGC | |||
intI1 | F | GGGTCAAGGATCTGGATTTCG | 1250 | [33] |
R | ACATGCGTGTAAATCATCGTCG | |||
sul1 | F | ATGGTGACGGTGTTCGGCATTCTGA | 900 | [34] |
R | TCTGGCTCCCAATCTAGTACGGATC | |||
qacEΔ1 | F | ATCGCAATAGTTGGCGAAGT | 600 | [35] |
R | CAAGCTTTTGCCCATGAAGC | |||
aadA | F | ACATCATTCCGTGGCGTTATC | 1100 | This study |
R | TTATTTGCCGACTACCTTGGTGA | |||
tnpA | F | GGCGGGATCTGCTTGTAGAG | 920 | [36] |
R | CTCCGGAGATGTCTGGCTTACT | |||
aad B | F | TCACAGCCAAACTATCAGG | 857 | This study |
R | TGCTCCACCAATCACAAT | |||
aacA4 | F | TGACCAACAGCAACGATTCC | 800 | [37] |
R | TTAGGCATCACTGCGTGTTC | |||
bla OXA | F | ATGAAAAACACAATACATATCAACTTCGC | 900 | This study |
R | GTGTGTTTAGAATGGTGATCGCATT | |||
bla KPC | F | TGTCACTGTATCGCCGTC | 1000 | [31] |
R | CTCAGTGCTCTACAGAAAACC | |||
bla TEM | F | CTGTCTATTTCGTTCATCC | 1061 | [24] |
R | CTCAGTATTGCCCGCTCC | |||
bla VIM | F | CACGAACCCAGTGGACATA | 1512 | This study |
R | TCACAGTAACCAGCAAATCA | |||
bla IMP | F | GCACGAACCCAGTTGACATA | 1394 | This study |
R | GTTTCTTCTTCCCACCATCC | |||
tniC | F | GCTCTGGTTGAGTTGGTG | 993 | This study |
R | GGATCCTTCCGCCTGTTG | |||
dfr | F | GTGAAAATATCACTAATGG | 750 | [38] |
R | TTAACCCCTTTGCCAGATTTG | |||
arr-3 | F | GGTGACTTGCTAACCACAG | 450 | This study |
R | ACAGTGACATAGCAAGTTCAG | |||
catB | F | CCTGAAGATTGCCAAGAGTGGT | 980 | [39] |
R | AGTTTGTTCAGGGTGACGAAGG | |||