Genetic characterization of novel class 1 Integrons In0, In1069 and In1287 to In1290, and the inference of In1069-associated integron evolution in Enterobacteriaceae

Table 1 Oligonucleotide primers used in this study

Target	Primer	Primer sequence (5′-3′)	Amplicon length (bp)	Reference
bla _KPC	KPC-F	GTATCGCCGTCTAGTTCTGC	637	[26]
bla _KPC	KPC-R	GGTCGTGTTTCCCTTTAGCC	637	[26]
bla _IMP	IMP-F	GGAATAGAGTGGCTTAAYTCTC	232	[26]
bla _IMP	IMP-R	GGTTTAAYAAAACAACCACC	232	[26]
bla _NDM	NDM-F	GGTTTGGCGATCTGGTTTTC	621	[26]
bla _NDM	NDM-R	CGGAATGGCTCATCACGATC	621	[26]
bla _VIM	VIM-F	GATGGTGTTTGGTCGCATA	390	[26]
bla _VIM	VIM-R	CGAATGCGCAGCACCAG	390	[26]
bla _OXA-23	OXA-23-F	GATCGGATTGGAGAACCAGA	501	[27]
bla _OXA-23	OXA-23-R	ATTTCTGACCGCATTTCCAT	501	[27]
bla _OXA-48	OXA-48-F	TTGGTGGCATCGATTATCGG	744	[28]
bla _OXA-48	OXA-48-R	GAGCACTTCTTTTGTGATGGC	744	[28]
bla _OXA-58	OXA-58-F	AAGTATTGGGGCTTGTGCTG	599	[27]
bla _OXA-58	OXA-58-R	CCCCTCTGCGCTCTACATAC	599	[27]
aacA4	aacA4-F	TATGAGTGGCTAAATCGAT	395	[23]
aacA4	aacA4-R	CCCGCTTTCTCGTAGCA	395	[23]
intI1	intI1-F	GCTGAAAGGTCTGGTCATAC	515	This study
intI1	intI1-R	GTTCTTCTACGGCAAGGTG	515	This study
tniR	tniR-F	CCAGGGTTGGCTGCTTGC	375	This study
tniR	tniR-R1	ATCGCCCACCTTGGTTTCC	375	This study
intI1 to tniR	intI1-F	GCTGAAAGGTCTGGTCATAC	Variable	This study
intI1 to tniR	tniR-R2	ACGCTGATCGTGTGGAAG	Variable	This study

ISSN: 2047-2994