Fig. 2

Detection of ^blaSHV, blaTEM and ^blaCTX-M-G (1,2,8,9, &25) antibiotic resistance genes in 110 ESBL Enterobacteriaceae pathogens isolated from children with UTIs. Representative samples are shown. Multiplex PCR was performed for detection of CTX-M groups while monoplex PCR was used for detection of TEM and SHV. The amplification products of each isolate were run on the same lane for detection of bla genes. Lane 1: ^blaTEM, blaSHV& ^blaCTX-MG1; Lane 2: ^blaTEM, ^blaSHV & bla CTX-M-G1: Lane 3: ^blaCTXM-G9; Lane 4: ^blaSHV & ^blaCTXM-G1; Lane 5: ^blaSHV; Lane6: ^blaCTX-MG1; Lane 7: ^blaCTXM-G2; Lane 8; ^blaCTXM-G8, Lane 9: ^blaTEM &^blaCTXM-G8; Lane 10, ^bla TEM, ^blaSHV &^blaCTXM-G8; Lane 11: ^blaSHV & ^blaCTXM-G1; Lane 12: ^blaNCTC 13,351 E. coli Positive control for ^blaTEM, NCTC 13368 K. pneumonia positive control for ^blaSHV, NCTC 13461 E. coli positive control for ^blaCTX-MG1: Lane 13: ATCC 25922 E. coli negative control