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Table 1 Primers and cycling conditions for the PCR assays

From: Antibiotic resistance, virulence factors and genotyping of Uropathogenic Escherichia coli strains

Gene

Primer sequence

Size (bp)

Cycling condition

Reference

hlyA

F: AACAAGGATAAGCACTGTTCTGGCT

R: ACCATATAAGCGGTCATTCCCGTCA

1177

1 cycle of 95 °C for 5 min;30 cycles of 94 °C for 1 min, 64 °C for 1 min, 72 °C for 1 min; 72 °C for 8 min final extension

[58]

FimH

F:GAGAAGAGGTTTGATTTAACTTATTG

R: AGAGCCGCTGTAGAACTGAGG

559

1 cycle of 95 °C for 5 min;30 cycles of 94 °C for 1 min, 60 °C for 1 min, 72 °C for 1 min; 72 °C for 8 min final extension

[60]

aer

F: GCTGGGCAGCAAACTGATAACTCTC

R: CATCAAGCTGTTTGTTCGTCCGCCG

602

1 cycle of 95 °C for 5 min;30 cycles of 94 °C for 1 min, 62 °C for 1 min, 72 °C for 1 min; 72 °C for 8 min final extension

[58]