From: Antibiotic resistance, virulence factors and genotyping of Uropathogenic Escherichia coli strains
Gene | Primer sequence | Size (bp) | Cycling condition | Reference |
---|---|---|---|---|
hlyA | F: AACAAGGATAAGCACTGTTCTGGCT R: ACCATATAAGCGGTCATTCCCGTCA | 1177 | 1 cycle of 95 °C for 5 min;30 cycles of 94 °C for 1 min, 64 °C for 1 min, 72 °C for 1 min; 72 °C for 8 min final extension | [58] |
FimH | F:GAGAAGAGGTTTGATTTAACTTATTG R: AGAGCCGCTGTAGAACTGAGG | 559 | 1 cycle of 95 °C for 5 min;30 cycles of 94 °C for 1 min, 60 °C for 1 min, 72 °C for 1 min; 72 °C for 8 min final extension | [60] |
aer | F: GCTGGGCAGCAAACTGATAACTCTC R: CATCAAGCTGTTTGTTCGTCCGCCG | 602 | 1 cycle of 95 °C for 5 min;30 cycles of 94 °C for 1 min, 62 °C for 1 min, 72 °C for 1 min; 72 °C for 8 min final extension | [58] |