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Table 1 Strains and plasmids used in this study

From: Spread of the florfenicol resistance floR gene among clinical Klebsiella pneumoniae isolates in China

Strain or plasmid

Relevant characteristic(s)a

Reference or source

Strain

 KP1 - KP23

23 strains carrying the floR gene from 328 clinically isolated K. pneumoniae samples

this study

 JM109

Escherichia coli JM109 was used as a host for the PCR product cloning

 

 EC600

Escherichia coli C600 was used as a host in conjugation experiments; Rfr

 

 ATCC25922

Escherichia coli ATCC25922 is an FDA clinical isolate

 

E. coli carrying plasmid

 pMD18-floRs/JM109

JM109 carrying the pMD18 vector encoding floR gene regions from 22 floR gene-positive strains

This study

 pKP5/EC600

The transconjugant with KP5 plasmid transferred into EC600

This study

 pKP6/EC600

The transconjugant with KP6 plasmid transferred into EC600

This study

 pKP18/EC600

The transconjugant with KP18 plasmid transferred into EC600

This study

Plasmid

 pKP18–125

KP18 plasmid transferred into EC600 by conjugation and sequenced

This study

 pMD18

Cloning vector for the PCR products of floR genes; Apr

TaKaRa

  1. aRf rifampin, Ap ampicillin