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Table 2 Growth conditions for S. aureus and P. aeruginosa biofilms

From: Hydrogen peroxide and sodium hypochlorite disinfectants are more effective against Staphylococcus aureus and Pseudomonas aeruginosa biofilms than quaternary ammonium compounds

Bacteria Strain

Hotplate Stirrer Settings

Test Culture preparation

Batch phase growth medium 24 h

CSTRa growth medium 24 h

S. aureus ATCC-6538

60 ± 5 rpm at 36 ± 1 °C

Frozen stock with 10 ml TSB (30 g TSB/L) overnight at 36 ± 1 °C

3 g/L TSB

1 g/L TSB

P. aeruginosa ATCC-15442

125 ± 5 rpm at 21 ± 2 °C

Frozen stock with 10 mL TSB (300 mg TSB/L) overnight at 36 ± 1 °C

300 mg/L TSB

100 mg/L TSB

  1. a Continuously stirred tank reactor (CSTR) phase