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Table 2 Growth conditions for S. aureus and P. aeruginosa biofilms

From: Hydrogen peroxide and sodium hypochlorite disinfectants are more effective against Staphylococcus aureus and Pseudomonas aeruginosa biofilms than quaternary ammonium compounds

Bacteria Strain Hotplate Stirrer Settings Test Culture preparation Batch phase growth medium 24 h CSTRa growth medium 24 h
S. aureus ATCC-6538 60 ± 5 rpm at 36 ± 1 °C Frozen stock with 10 ml TSB (30 g TSB/L) overnight at 36 ± 1 °C 3 g/L TSB 1 g/L TSB
P. aeruginosa ATCC-15442 125 ± 5 rpm at 21 ± 2 °C Frozen stock with 10 mL TSB (300 mg TSB/L) overnight at 36 ± 1 °C 300 mg/L TSB 100 mg/L TSB
  1. a Continuously stirred tank reactor (CSTR) phase