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Table 4 Genotypic characterization of NDM-positive isolates co-transferring blaNDM-1 and PMQRs

From: Evaluation of co-transfer of plasmid-mediated fluoroquinolone resistance genes and blaNDM gene in Enterobacteriaceae causing neonatal septicaemia

Strain no. Organism NDM PMQR Other Resistance genes MEM MIC CIP MIC Plasmid size Plasmid type Plasmid addiction system Integron
EN5132 Escherichia coli bla NDM-1 aac(6′)-Ib-cr bla CTX-M, TEM, OXA
armA
2 > 32 212, 6, 3 FIB,A/C,FIIK,FII,N PemK, CcdAB, Hok-Sok intI1
EN5132.T1 E. coli J53 Fox-azide bla NDM-1 aac(6′)-Ib-cr bla CTX-M, TEM, OXA
armA
2 0.047 212 A/C intI1
EN5127 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr, qnrS aac(6′)-Ib, bla SHV, rmtC 24 > 32 210, 4,3 A/C,FIIK,N intI1
EN5127.T1 E. coli J53 Fox-azide bla NDM-1 aac(6′)-Ib-cr aac(6′)-Ib, rmtC 2 0.016 210 N, A/C IntI1
EN5127.T2 E. coli J53 Cip-Azide bla NDM-1 aac(6′)-Ib-cr aac(6′)-Ib, rmtC 6 0.5 210 N, A/C IntI1
EN5150 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB aac(6′)-Ib, bla CTX-M, SHV, OXA 4 > 32 248 FIIK, PemK IntI1
EN5150.T1 E. coli J53 Fox-azide bla NDM-1 aac(6′)-Ib-cr,qnrB aac(6′)-Ib bla CTX-M, OXA 4 0.25 248 FIIK, IntI1
EN5150.T2 E. coli J53 Cip-Azide bla NDM-1 aac(6′)-Ib-cr,qnrB aac(6′)-Ib bla CTX-M, OXA 4 0.25 248 FIIK,   IntI1
EN5151 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB aac(6′)-Ib bla CTX-M, SHV,OXA 2 > 32 248, 128 FIIK, IntI1
EN5151.T1 E. coli J53 Fox-azide bla NDM-1 qnrB aac(6′)-Ib, 1 0.064 248 FIIK, IntI1
EN5151.T2 E. coli J53 Cip-Azide bla NDM-1 qnrB aac(6′)-Ib 0.5 0.047 248 FIIK,   IntI1
EN5163 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, SHV, OXA, aac(6′)-Ib 6 > 32 266, 154 FIIK IntI1
EN5163.T1 E. coli J53 Fox-azide bla NDM-1 qnrB aac(6′)-Ib 3 0.125 266 FIIK IntI1
EN5163.T2 E. coli J53 Cip-Azide bla NDM-1 qnrB aac(6′)-Ib 2 0.125 266 FIIK   IntI1
EN5165 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB aac(6′)-Ib bla CTX-M, SHV, OXA 12 > 32 266, 154 FIIK IntI1
EN5165.T1 E. coli J53 Fox-azide bla NDM-1 qnrB aac(6′)-Ib 0.38 0.75 266 FIIK   IntI1
EN5165.T2 E. coli J53 Cip-Azide bla NDM-1 qnrB aac(6′)-Ib 2 0.064 266 FIIK   IntI1
EN5166 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB aac(6′)-Ib bla CTX-M, SHV, OXA 4 > 32 266, 154 FIIK IntI1
EN5166.T1 E. coli J53 Fox-azide bla NDM-1 qnrB aac(6′)-Ib 0.5 0.047 266 FIIK IntI1
EN5166.T2 E. coli J53 Cip-Azide bla NDM-1 qnrB bla CTX-M, OXA
aac(6′)-Ib
3 0.064 266 FIIK   IntI1
EN5170 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr, qnrB aac(6′)-Ib, bla CTX-M, SHV, OXA 4 > 32 260, 200 FIIK IntI1
EN5170.T1 E. coli J53 Fox-azide bla NDM-1 qnrB aac(6′)-Ib, 0.5 0.064 260 FIIK IntI1
EN5170.T2 E. coli J53 Cip-Azide bla NDM-1 qnrB aac(6′)-Ib 0.5 0.25 260 FIIK   IntI1
EN5173 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, SHV, OXA
aac(6′)-Ib
1.5 > 32 116, 90 FIIK, IntI1
EN5173.T1 E. coli J53 Fox-azide bla NDM-1 qnrB aac(6′)-Ib 0.5 0.094 116 FIIK IntI1
EN5173.T2 E. coli J53 Cip-Azide bla NDM-1 qnrB aac(6′)-Ib 1 0.047 116 FIIK   IntI1
EN5174 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, SHV,OXA , armA 3 16 260, 7, 5, 4 FIIK, N, HIB-M VagC/D IntI1
EN5174.T1 E. coli J53 Fox-azide bla NDM-1 aac(6′)-Ib-cr,qnrB blaCTX-M, SHV,OXA, armA 3 6 260, 7, 5, 4 FIIK, N, HIB-M Vagc/D IntI1
EN5181 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrS bla CTX-M, SHV, TEM, 2 14 220 FIIK, FII PemK
EN5181.T1 E. coli J53 Fox-azide bla NDM-1 qnrS bla CTX-M, TEM 0.5 0.19 220 FIIK
EN5181.T2 E. coli J53 Cip-Azide bla NDM-1 qnrS bla CTX-M, TEM 0.75 0.19 220 FIIK
EN5185 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, SHV, OXA
aac(6′)-Ib
3 > 32 266, 154 FIIK IntI1
EN5185.T1 E. coli J53 Fox-azide bla NDM-1 aac(6′)-Ib-cr,qnrB aac(6′)-Ib 0.5 0.032 266 FIIK IntI1
EN5185.T2 E. coli J53 Cip-Azide bla NDM-1 aac(6′)-Ib-cr,qnrB aac(6′)-Ib 1.5 0.19 266 FIIK  - IntI1
  1. MEM: meropenem, CIP: ciprofloxacin, (-): absent or untypable (in case of plasmid types), E. coli J53 Fox-azide or ‘.T1’ : transconjugants selected in cefoxitin (10μg/ml)-sodium azide (100μg/ml), ), E. coli J53 Cip-Azide or ‘.T2’ : transconjugants selected in ciprofloxacin (0.06 μg/ml)-sodium azide (100 μg/ml)