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Table 5 Genotypic characterization of NDM-positive isolates transferring only blaNDM gene

From: Evaluation of co-transfer of plasmid-mediated fluoroquinolone resistance genes and blaNDM gene in Enterobacteriaceae causing neonatal septicaemia

Strain no. Organism NDM PMQR Other Resistance genes MEM MIC CIP MIC Plasmid size in kb (approximately) Plasmid type (Inc) Plasmid addiction system Integron
EN5134 Escherichia coli bla NDM-15 aac(6′)-Ib-cr bla CTX-M, TEM, OXA
rmtB
24 > 32 225, 158,4 FIA, FII, I1A PemK, CcdAB, Hok-Sok, PndCA intI1
EN5134.T1 E. coli J53 Fox-azide bla NDM-15 bla TEM, rmtB 2 0.012 121 FII intI1
EN5141 Escherichia coli bla NDM-1 aac(6′)-Ib-cr bla CTX-M, TEM, OXA, armA 4 > 32 246, 134, 6 FIB, HIB-M, FII PemK, Hok-Sok
EN5141.T1 E. coli J53 Fox-azide bla NDM-1 bla TEM, 1.5 0.008 246, 6 HIB-M
EN5143 Escherichia coli bla NDM-1 bla TEM
rmtB
4 > 32 105,56, 9 FIA, I1γ, FII, I1a PndC/A intI1
EN5143.T1 E. coli J53 Fox-azide bla NDM-1 bla TEM
rmtB
1.5 0.008 105 FIA, FII intI1
EN5169 Escherichia coli bla NDM-7 bla TEM
rmtB
> 32 > 32 200, 5, 2 I1A, FIA, FIB, FIIS, R, FII PndCA, Hok-Sok, ccdAB, SrnBC, PemK -
EN5169.T1 E. coli J53 Fox-azide bla NDM-7 bla TEM, 1 0.012 200,5 I1A, Pnd C/A -
EN5177 Escherichia coli bla NDM-5 bla TEM
rmtB
4 > 32 163,126,55,5, 2 FIB, FIIS PemK IntI1
EN5177.T1 E. coli J53 Fox-azide bla NDM-5 bla TEM
rmtB
1 0.012 163,126 FIB, FIIS IntI1
EN5114 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr bla CTX-M, SHV, OXA
armA
1.5 1.5 248 HIB-M PemK intI1
EN5114.T1 E. coli J53 Fox-azide bla NDM-1 bla CTX-M
armA
1.5 0.012 248,180 HIB-M -
EN5117 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr bla CTX-M
armA
1.5 1.5 248 HIB-M PemK intI1
EN5117.T1 E. coli J53 Fox-azide bla NDM-1 bla CTX-M, SHV, OXA
armA
1 0.016 248 HIB-M -
EN5123 Klebsiella pneumoniae bla NDM-1 bla CTX-M, aac(6′)-Ib 3 0.094 210, 20,7,5 FIA, R, FIIK intI1
EN5123.T1 E. coli J53 Fox-azide bla NDM-1 bla CTX-M, aac(6′)-Ib 1 0.008 210,5 FIIK -
EN5129 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, aac(6′)-Ib 2 12 263,230,15.6,6.7, FIIK, R intI1
EN5129.T1F-A E. coli DH10B - aac(6′)-Ib-cr,qnrB bla CTX-M 0.023 0.19 230 FIIK -
EN5130 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr bla CTX-M, OXA 8 > 32 270,205,29,13, 8, 7, 6,5, 3, 2 FIIK, FIA, X2 intI1
EN5130.T1F E. coli DH10B bla NDM-1 bla CTX-M, OXA
-
2 < 0.002 270, 205, 29, 13, 8, 7,5, 3, 2 -
EN5135 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, TEM, SHV, OXA 1.5 16 230, 5, 4, 2, 0.1 FIIK, HIB-M VagCD, intI1
EN5135.T1 E. coli J53 Fox-azide bla NDM-1 bla CTX-M 1.5 0.004 230 HIB-M
EN5136 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, TEM, OXA 3 4 340, 212, 9, 6, 4 FIIK, HIB-M PemK, CcdAB, VagCD, Hok-Sok intI1
EN5136.T1 E. coli J53 Fox-azide bla NDM-1 bla CTX-M, TEM 0.75 0.012 340 HIB-M intI1
EN5137 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, TEM, OXA 1.5 32 310,162, 8, 6, 4 FIIK, HIB-M PemK, CcdAB, VagCD, Hok-Sok intI1
EN5137.T1 E. coli J53 Fox-azide bla NDM-1 bla CTX-M, TEM 0.75 0.012 310 HIB-M -
EN5139 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, TEM, OXA 1 16 250,112,5, 4 FIIK, HIB-M PemK, VagCD intI1
EN5139.T1 E. coli J53 Fox-azide bla NDM-1 bla CTX-M, TEM, 1.5 0.23 250 HIB-M -
EN5142 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, TEM, OXA
rmtC
8 2 248,104 FIIK, FIB-M intI1
EN5142.T1 E. coli J53 Fox-azide bla NDM-1 -
rmtC
1.5 0.008 248, 104 intI1
EN5144 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, SHV, OXA
armA
2 6 227, 52, 5, 4 HIB-M -
EN5144.T1 E. coli J53 Fox-azide bla NDM-1 bla CTX-M, SHV, OXA
armA
0.5 0.016 227 HIB-M -
EN5146 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr bla CTX-M, rmtC, aac(6′)-Ib 10 > 32 192,164, 7 FIA, A/C, FIIK -
EN5146.T1F E. coli DH10B bla NDM-1 bla CTX-M, aac(6′)-Ib 6 < 0.002 164 A/C -
EN5154 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr bla CTX-M
armA
8 > 32 241, 201, 34, 29,8, 6, 4,3 FIIS, R, FIIK PemK, VagCD IntI1
EN5154.T1F E. coli DH10B bla NDM-1 bla CTX-M, 6 0.012 240, 4 FIIS IntI1
EN5175 Klebsiella pneumoniae bla NDM-1 aac(6′)-Ib-cr, qnrB bla CTX-M, TEM, OXA
armA
3 4 123, 88,7,5,3 N, FIIK, HIB-M PemK, VagCD IntI1
EN5175.T1 E. coli J53 Fox-azide bla NDM-1 bla CTX-M, SHV, OXA
armA
1.5 0.012 123 HIB-M - -
EN5175.T2 E. coli J53 Cip-Azide qnrB armA 0.023 0.125 96, 50 N, FIIK VagCD -
EN5180 Klebsiella pneumoniae bla NDM-1 armA,rmtC, bla TEM
aac(6′)-Ib
32 > 32 293,208,5,4 A/C, FIIK, FIB-M, HIB-M VagCD IntI1
EN5180.T1 E. coli J53 Fox-azide bla NDM-1 bla TEM
rmtC
1.5 0.016 208 A/C   IntI1
EN5186 Klebsiella pneumoniae bla NDM-1 qnrB armA, rmtC 6 > 32 235,188,47,5, 3, FII IntI1,IntI2
EN5186.T1 E. coli J53 Fox-azide bla NDM-1 bla CTX-M, TEM
rmtC
1.5 0.008 188 - - IntI1
EN5131 Enterobacter aerogenosa bla NDM-1 aac(6′)-Ib-cr,qnrB bla CTX-M, OXA, rmtC 4 4 205,27,17,7,5 ccdA/B, hok -sok intI1
EN5131.T1 E. coli DH10B bla NDM-1 bla CTX-M, rmtC 2 < 0.002 205,27,7  
  1. MEM: meropenem, CIP: ciprofloxacin, (-) : absent or untypable (in case of plasmid types), E. coli J53 Fox-azide or ‘.T1’ : transconjugants selected in cefoxitin (10μg/ml)-sodium azide (100μg/ml), E. coli DH10B or ‘.TF’: Transformants selected in cefoxitin (5μg/ml) , ‘.TF-A : Transformants selected in ampicillin (50μg/ml), E. coli J53 Cip-Azide or ‘.T2’ : transconjugants selected in ciprofloxacin (0.06 μg/ml)-sodium azide (100 μg/ml)