Table 1 Overview of primers
Primers | Sequence 5’-3’ | Melting point |
|---|---|---|
CIT-F1 CIT-F2 CIT-R1 CIT-R2 CIT-R3 | TGG CCA GAA CTG ACA GGC AAA TGG CCT GAA CTG ACT GGT AAG TTT CTC CTG AAC GTG GCT GGC TTT CTC CTG AAC GCG GCT GGC TTT CTC CTG AAC CTG GCT GGC | 87.840C |
MOX-F MOX-R1 MOX-R2 | GCT GCT CAA GGA GCA CAG GAT CAC ATT GAC ATA GGT GTG GTG C CAC ATT GAG GTA GGT ATG GTA C | 91.040C |
FOX-F FOX-R | AAC ATG GGG TAT CAG GGA GAT G CAA AGC GCG TAA CCG GAT TGG | 89.150C |
DHA-F DHA-R | AAC TTT CAC AGG TGT GCT GGG T GCT GCC ACT GCT GAT AGA A | 88.230C |
ACC-F ACC-R | GTG CAA GCC AAT ATG GGG CAG CTC CCA CAT CAG ATC CTG AGT | 83.580C |
EBC-F1 EBC-F2 EBC-F3 EBC-R1 EBC-R2 EBC-R3 | TCG GTA AAG CCG ATG TTG CGG TTG GCA AAG CCG ATA TCG CGG TTG GCA AGG CCG ATA TCG CGG CTT CCA CTG CGG CTG CCA GTT CGC CCA CTG TGG TTG CCA GGA CTT CCA CTG CGG TTG CCA GTG | 89.500C |