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Table 1 Materials and technological parameters of Various formulation

From: Doxycycline-encapsulated solid lipid nanoparticles as promising tool against Brucella melitensis enclosed in macrophage: a pharmacodynamics study on J774A.1 cell line

  sample Lipid (mg) Lipid surfactant (ML, Mg) Water surfactant (ML, Mg) Water added (ML) PS (nm) PDI Zeta potential (mV) EE (%) DL (%)
Before lyophilization SLN1 without doxycycline Palm oil (600) Lecithin (60) Tween 80 (6) 50 251 ± 15 0.277 ± 0.021 −31.6 ± 1.4 95.4 ± 3.5 11.3 ± 1.1
SLN 1 with doxycycline Palm oil (600) Lecithin (60) Tween 80 (6) 50 265 ± 23 0.284 ± 0.012 −29.3 ± 2.1
SLN 2 without doxycycline Stearic acid (600) Span 80 (6) Poloxamer407 (60) 100 456 ± 21 0.429 ± 0.032 −11.6 ± 4.4 93.7 ± 5.3 8.9 ± 2.3
SLN 2 with doxycycline Stearic acid (600) Span 80 (6) Poloxamer407 (60) 100 465 ± 23 0.344 ± 0.012 −14.3 ± 3.5
SLN 3 without doxycycline Palm oil (1200) Span 80 (6) Tween 80 (3) 100 358 ± 39 0.536 ± 0.019 −9.6 ± 1.7 94.1 ± 7.1 9.2 ± 3.8
SLN 3 with doxycycline Palm oil (1200) Span 80 (6) Tween 80 (3) 100 375 ± 42 0.591 ± 0.010 −11.3 ± 2.6
SLN 4 without doxycycline Stearic acid (1200) Lecithin (60) Tween 80 (3) 50 491 ± 25 0.447 ± 0.033 −18.6 ± 1.4 97.4 ± 4.5 10.7 ± 1.4
SLN 4 with doxycycline Stearic acid (1200) Lecithin (60) Tween 80 (3) 50 415 ± 59 0.494 ± 0.093 −19.3 ± 1.2
After lyophilization SLN 1 without doxycycline Palm oil (600) Lecithin (60) Tween 80 (6) 50 294 ± 27 0.281 ± 0.044 −30.7 ± 2.04 94.9 ± 3.2 11.2 ± 1.3
SLN 1 with doxycycline Palm oil (600) Lecithin (60) Tween 80 (6) 50 299 ± 34 0.290 ± 0.027 −28.7 ± 3.2
SLN 2 without doxycycline Stearic acid (600) Span 80 (6) Poloxamer407 (60) 100 491 ± 33 0.499 ± 0.042 −12.6 ± 3.5 93.9 ± 4.7 8.2 ± 1.9
SLN 2 with doxycycline Stearic acid (600) Span 80 (6) Poloxamer407 (60) 100 471 ± 34 0.412 ± 0.012 −14.3 ± 3.5
SLN 3 without doxycycline Palm oil (1200) Span 80 (6) Tween 80 (3) 100 378 ± 28 0.591 ± 0.053 −17.6 ± 2.1 91.3 ± 2.7 8.8 ± 3.1
SLN 3 with doxycycline Palm oil (1200) Span 80 (6) Tween 80 (3) 100 399 ± 39 0.612 ± 0.125 −15.3 ± 1.7
SLN 4 without doxycycline stearic acid (1200) Lecithin (60) Tween 80 (3) 50 511 ± 45 0.493 ± 0.325 −17.8 ± 1.7 96.1 ± 3.9 9.9 ± 1.7
SLN 4 with doxycycline stearic acid (1200) Lecithin (60) Tween 80 (3) 50 575 ± 39 0.524 ± 0.987 −16.3 ± 2.2
  1. PS particle size, PDI polydispersity index, EE entrapment efficiency, DL drug loading