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Table 3 Results of well diffusion and MIC test

From: Doxycycline-encapsulated solid lipid nanoparticles as promising tool against Brucella melitensis enclosed in macrophage: a pharmacodynamics study on J774A.1 cell line

Methods

Antibacterial activity

Well Diffusion

Zone of inhibition (mm) in three time (h) and three concentration(μg/ml)

Doxycycline

(50-25-12.5 μg/ml)

DOX-SLN

(50-25-12.5 μg/ml)

Free SLN (blank)

(50-25-12.5 μg/ml)

24 h

48 h

72 h

24 h

48 h

72 h

24 h

48 h

72 h

35

30

22

35

30

22

35

30

22

21

19

15

26

24

20

31

30

22

0

0

0

0

0

0

0

0

0

MIC

MIC value (μg/ml)

doxycycline

Dox-SLN

free SLN (blank)

24 h

24 h

48 h

72 h

24 h

48 h

72 h

0.5

25

12.5

6.25

-

-

-