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Table 1 PCR conditions of amplification

From: Phenotypic and genotypic characterization of carbapenem-resistant Acinetobacter baumannii isolates from Egypt

Carbapenemase class/Insertion sequence Gene Thermal cycling conditions Master-mix used
Initial denaturation 30 cycles Final extension
Denaturation Annealing Extension
Class D carbapenemases [41] blaoxa-51
blaoxa-23
blaoxa-58
blaoxa-40
95 °C /1 min 95 °C /15 s 52 °C /15 s 72 °C /10 s 72 °C /10 min MyTaq™ HS Mix
Class B carbapenemases [42, 43] blaIMP 94 °C /5 min 94 °C /40 s 47 °C /1 min 72 °C /2 min 72 °C /5 min OnePCR™
blaVIM 94 °C /5 min 94 °C /40 s 51 °C /1 min
blaSIM
blaSPM
blaGIM
94 °C /5 min 94 °C /40 s 52 °C /1 min
blaNDM 94 °C /5 min 94 °C /40 s 54 °C /1 min
Class A carbapenemases [44] blaKPC 94 °C /5 min 94 °C /40 s 54 °C /1 min 72 °C /2 min 72 °C /5 min OnePCR™
Insertion sequences [17] ISAba1
ISAba2
ISAba3