Phenotypic and genotypic characterization of carbapenem-resistant Acinetobacter baumannii isolates from Egypt

Table 1 PCR conditions of amplification

Carbapenemase class/Insertion sequence	Gene	Thermal cycling conditions					Master-mix used
		Initial denaturation	30 cycles			Final extension
		Initial denaturation	Denaturation	Annealing	Extension	Final extension
Class D carbapenemases [41]	bla_oxa-51 bla_oxa-23 bla_oxa-58 bla_oxa-40	95 °C /1 min	95 °C /15 s	52 °C /15 s	72 °C /10 s	72 °C /10 min	MyTaq™ HS Mix
Class B carbapenemases [42, 43]	bla_IMP	94 °C /5 min	94 °C /40 s	47 °C /1 min	72 °C /2 min	72 °C /5 min	OnePCR™
	bla_VIM	94 °C /5 min	94 °C /40 s	51 °C /1 min
	bla_SIM bla_SPM bla_GIM	94 °C /5 min	94 °C /40 s	52 °C /1 min
	bla_NDM	94 °C /5 min	94 °C /40 s	54 °C /1 min
Class A carbapenemases [44]	bla_KPC	94 °C /5 min	94 °C /40 s	54 °C /1 min	72 °C /2 min	72 °C /5 min	OnePCR™
Insertion sequences [17]	ISAba1 ISAba2 ISAba3	94 °C /5 min	94 °C /40 s	54 °C /1 min	72 °C /2 min	72 °C /5 min	OnePCR™

ISSN: 2047-2994