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Table 4 Summary of the species identification, nucleotide differences range and amount of available reference sequences based on 16S rRNA, sodA, tuf and rpoB genes and 16S–23S rRNA region for Streptococcus genus

From: Development of a reference data set for assigning Streptococcus and Enterococcus species based on next generation sequencing of the 16S–23S rRNA region

 

16S rRNA gene

sodA gene

tuf gene

rpoB gene

NGS 16S–23S rRNA

Unambiguous species identification

25 species (60%)

34 species (81%)

39 species (93%)

39 species (93%)

39 species (93%)

The lowest amount of nucleotides differences

0

0

0

0

2

The highest amount of nucleotides differences

228

176

205

186

1209

No. of species without reference sequences in the databases

0

8

9

5

15