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Table 4 Summary of the species identification, nucleotide differences range and amount of available reference sequences based on 16S rRNA, sodA, tuf and rpoB genes and 16S–23S rRNA region for Streptococcus genus

From: Development of a reference data set for assigning Streptococcus and Enterococcus species based on next generation sequencing of the 16S–23S rRNA region

  16S rRNA gene sodA gene tuf gene rpoB gene NGS 16S–23S rRNA
Unambiguous species identification 25 species (60%) 34 species (81%) 39 species (93%) 39 species (93%) 39 species (93%)
The lowest amount of nucleotides differences 0 0 0 0 2
The highest amount of nucleotides differences 228 176 205 186 1209
No. of species without reference sequences in the databases 0 8 9 5 15