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Table 2 The efficiencies of the primers used in the development of the RT-qPCR study

From: The anti-virulence effect of cranberry active compound proanthocyanins (PACs) on expression of genes in the third-generation cephalosporin-resistant Escherichia coli CTX-M-15 associated with urinary tract infection

Primer

Efficiency %

16S (reference gene)

84.3

recA

111.1

CTX-M

99.7

sat

111.6

traT

94.3

chuA

115.6

kpsM

101.7

soxS

103.0

usp

90.2

hcaT

118.9

IhfB

115.3

  1. Primer efficiencies were calculated using serial dilutions of amplicons 1:1000. An efficiency of 80–120% was considered.