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Table 2 The efficiencies of the primers used in the development of the RT-qPCR study

From: The anti-virulence effect of cranberry active compound proanthocyanins (PACs) on expression of genes in the third-generation cephalosporin-resistant Escherichia coli CTX-M-15 associated with urinary tract infection

PrimerEfficiency %
16S (reference gene)84.3
recA111.1
CTX-M99.7
sat111.6
traT94.3
chuA115.6
kpsM101.7
soxS103.0
usp90.2
hcaT118.9
IhfB115.3
  1. Primer efficiencies were calculated using serial dilutions of amplicons 1:1000. An efficiency of 80–120% was considered.
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Antimicrobial Resistance & Infection Control

ISSN: 2047-2994

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