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Table 1 Minimal inhibitory concentration for various antibiotics

From: Development of amoxicillin resistance in Escherichia coli after exposure to remnants of a non-related phagemid-containing E. coli: an exploratory study

Isolate

MIC (mg/L)

D68C AmpC & ESBL Detection set conclusion

E-test®

Vitek®

amox

amcl

pita

cfrx

cfxt

cftz

cftx

AmpC hyperproduction¥

pBS-E12

> = 256

> = 32

> = 128

8

<=4

<=1

<=1

No

EUR1

6

<=2

<=4

4

<=4

<=1

<=1

Yes

EUR1M1

> = 256

> = 32

8

32

> = 64

4

<=1

Yes

EUR1M2

> = 256

> = 32

16

32

> = 64

4

<=1

Yes

EUR1M3

> = 256

> = 32

<=4

16

16

<=1

<=1

Yes

EUR1M4

> = 256

> = 32

8

32

32

<=1

<=1

Yes

EUR1M5

> = 256

> = 32

8

> = 64

> = 64

4

2

Yes

EUR1M6

256

> = 32

<=4

16

> = 64

<=1

<=1

Yes

EUR1M7

> = 256

> = 32

8

32

> = 64

4

<=1

Yes

EUR1M8

> = 256

> = 32

<=4

16

16

<=1

<=1

Yes

EUR1M9

256

> = 32

<=4

16

16

<=1

<=1

Yes

EUR1M10

128

> = 32

<=4

16

32

<=1

<=1

Yes

EUR1M11

128

> = 32

<=4

32

16

<=0.25

<=0.25

Yes

EUR1M12

> = 256

> = 32

16

32

16

2

1

Yes

EUR1M13

> = 256

> = 32

16

32

32

2

1

Yes

EUR1M14

> = 256

> = 32

16

32

16

1

<=0.25

Yes

EUR1M15

196

> = 32

<=4

16

16

0.5

<=0.25

Yes

EUR1M16

196

> = 32

<=4

16

16

0.5

<=0.25

Yes

EUR1M17

256

> = 32

<=4

32

32

2

0.5

Yes

EUR1M18

> = 256

> = 32

16

32

16

2

1

Yes

EUR1M19

> = 256

> = 32

8

32

16

2

<=0.25

Yes

EUR1M20

64

16

<=4

4

<=4

0.5

<=0.25

Yes

EUR1M21

> = 256

> = 32

16

32

16

4

1

Yes

EUR1M22

256

> = 32

16

32

16

4

1

Yes

EUR1S1

> = 256

> = 32

<=4

16

32

<=1

<=1

Yes

EUR1S2

> = 256

> = 32

8

32

> = 64

2

<=1

Yes

EUR1E1

> = 256

> = 32

<=4

16

32

<=1

<=1

Yes

EUR2

1

<=2

<=4

2

<=4

<=1

<=1

No

EUR2M1

> = 256

> = 32

8

8

<=4

<=1

<=1

Yes

  1. MICs were measured with ETEST® (bioMérieux, Marcy l’Etoile, France) (amoxicillin) or the VITEK 2® system (bioMérieux, Marcy l’Etoile, France). ¥Interpretation of D68C AmpC & ESBL Detection set according to manufacturers instruction. Zone diameters as measured in the D68C AmpC & ESBL Detection set are depicted in Supplementary table S3. amox: amoxicillin; amcl: amoxicillin-clavulanic acid; pita: piperacillin-tazobactam; cfrx: cefuroxime; cfxt: cefoxitin; cftz: ceftazidime; cftx: cefotaxime; mero: meropenem; imip: imipenem;M: amoxicillin-resistant mutant after exposure to remnants of the pBS-E12 and Sterillium®S: amoxicillin-resistant mutant after exposure to Sterillium® onlyE: amoxicillin-resistant mutant after exposure to E12 and Sterillium®