| E.coli K-12 (wildtype) | Base in wildtype cAmpC promoter/attenuator region | |
|---|
| −32 | − 23.1 | − 21.1 | −19 | − 18 | −16.1 | − 11 | + 58 | + 81 |
|---|
| T | – | – | T | G | – | C | C | G |
|---|
| Isolate | Position of mutation in cAmpC promoter/attenuator regiona | Date experiments performed¤ |
|---|
| −32 | −23.1 | −21.1 | −19 | −18 | −16.1 | −11 | + 58 | + 81 |
|---|
| pBS-E12¥ | | | | | | | | | | |
| E12 | | | | | | | | | | |
| EUR1 (wildtype) | | | | | | | | | A | |
| EUR1M1 | A | | | | | | | | A | 10th May |
| EUR1M2 | | G | C | A | | | | | A | 10th May |
| EUR1M3 | | G | C | A | | | | | A | 11th May |
| EUR1M4 | | G | C | A | | | | | A | 12th May |
| EUR1M5 | | G | C | A | | | | | A | 12th May |
| EUR1M6 | | | | | | | T | | A | 13th May |
| EUR1M7 | | G | C | A | | | | | A | 25th May |
| EUR1M8 | | G | C | A | | | | | A | 26th May |
| EUR1M9 | | G | C | A | | | | | A | 1st June |
| EUR1M10 | | | | | | | T | | A | 1st June |
| EUR1S1 | | | T | | | | | | A | 23th May |
| EUR1S2 | A | | | | | | | | A | 17th August |
| EUR2 (wildtype) | | | | | A | | | T | | |
| EUR2M1 | | | | | A | A | | T | | 12th May |
- aPosition numbering of the cAmpC promoter/attenuator region as defined by Mulvey et al. [31]. In position number n.x: decimal number x refers to an insertion at position n. Positions −32 and − 11 are part of the wild-type promoter boxes. Position − 19 is part of the alternate promoter box. Positions − 21.1 and − 23.1 are part of the spacer region of both the wild-type and alternate promoter. Position − 16.1 is part of the spacer region of the wild-type promoter. ¥ strain used in experiments on 1st of June. ¤ all experiments were performed in 2016
