Development of amoxicillin resistance in Escherichia coli after exposure to remnants of a non-related phagemid-containing E. coli: an exploratory study

Table 2 Mutations in cAmpC promoter/attenuator region of the wildtype and mutant isolates

E.coli K-12 (wildtype)	Base in wildtype cAmpC promoter/attenuator region
	−32	− 23.1	− 21.1	−19	− 18	−16.1	− 11	+ 58	+ 81
	T	–	–	T	G	–	C	C	G
Isolate	Position of mutation in cAmpC promoter/attenuator region^a									Date experiments performed^¤
Isolate	−32	−23.1	−21.1	−19	−18	−16.1	−11	+ 58	+ 81	Date experiments performed^¤
pBS-E12^¥
E12
EUR1 (wildtype)									A
EUR1M1	A								A	10th May
EUR1M2		G	C	A					A	10th May
EUR1M3		G	C	A					A	11th May
EUR1M4		G	C	A					A	12th May
EUR1M5		G	C	A					A	12th May
EUR1M6							T		A	13th May
EUR1M7		G	C	A					A	25th May
EUR1M8		G	C	A					A	26th May
EUR1M9		G	C	A					A	1st June
EUR1M10							T		A	1st June
EUR1S1			T						A	23th May
EUR1S2	A								A	17th August
EUR2 (wildtype)					A			T
EUR2M1					A	A		T		12th May

^aPosition numbering of the cAmpC promoter/attenuator region as defined by Mulvey et al. [31]. In position number n.x: decimal number x refers to an insertion at position n. Positions −32 and − 11 are part of the wild-type promoter boxes. Position − 19 is part of the alternate promoter box. Positions − 21.1 and − 23.1 are part of the spacer region of both the wild-type and alternate promoter. Position − 16.1 is part of the spacer region of the wild-type promoter. ¥ strain used in experiments on 1st of June. ¤ all experiments were performed in 2016

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