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Table 1 Principal oligonucleotide primers used in this study

From: Emerging coexistence of three PMQR genes on a multiple resistance plasmid with a new surrounding genetic structure of qnrS2 in E. coli in China

Primer

Primer sequence (5′ → 3′)

Amplicon size (bp)

Parameters of PCR

P1

TGCTGTGCTCGTCTAAT

3531

(95 °C, 5 min) + {(95 °C, 30 s) + (60 °C, 30 s) + (72 °C, 3 min 35 s)} × 34 + (72 °C, 5 min)

P2

AATGCCTGGCGTGTTT

P3

TGGCTATCACAACAAGG

1190

(95 °C, 5 min) + {(95 °C, 30 s) + (60.5 °C, 30 s) + (72 °C, 1 min 15 s)} × 29 + (72 °C, 5 min)

P4

TTAGAACTAGGGCAGGA

P5

CGCTTAACTAAGTGCAAGAA

1008

(95 °C, 5 min) + {(95 °C, 30 s) + (61.2 °C, 30 s) + (72 °C, 1 min 5 s)} × 29 + (72 °C, 5 min)

P6

GCTACGCTGAGAACCATC