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Table 1 Vancomycin resistant enterococci typing methods and accompanying characteristics

From: Enterococcus faecium: from microbiological insights to practical recommendations for infection control and diagnostics

Method MLVA MLST PFGE cgMLST WGS Transposon analysis
Principle Fragment length of variable tandem repeat loci Sequences of multiple house keeping genes DNA based macro restriction analysis Genome-wide gene-by-gene approach of 1423 genes on allelic level Whole genome analysis Sequences of transposon content and integration
Reproducibility High High Medium Excellent Excellent Excellent
Ease of performance Very easy Easy Laborious Easy Easy Easy
Data interpretation Easy-moderate Easy Difficult Easy Various Moderate
Ease of data exchange Easy Easy Difficult Easy Possible Possible
Costs Low Medium Medium High, extracted from WGS High High, extracted from WGS
Discriminatory power Low Medium High Excellent Excellent Additional
  1. MLVA Multiple Locus Variable Number of Tandem Repeat Analysis, MLST Multi-locus Sequence Typing, PFGE Pulsed-field gel electrophoresis, cgMLST core-genome MLST, WGS whole-genome sequencing