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Fig. 5 | Antimicrobial Resistance & Infection Control

Fig. 5

From: The rapid and visual detection of methicillin-susceptible and methicillin-resistant Staphylococcus aureus using multiplex loop-mediated isothermal amplification linked to a nanoparticle-based lateral flow biosensor

Fig. 5

Sensitivity analysis of m-LAMP detection with serial dilutions of genomic DNA extracted from the MRSA strain. Two sets of primers targeting the femA and mecA genes were simultaneously added to a reaction vessel, and the LoD of m-LAMP for detecting S. aureus and identifying MRSA was analyzed with a lateral flow biosensor. Biosensors 1–8 represent the genomic DNA (methicillin-resistant S. aureus, ATCC43300) amounts of 10 ng, 10 pg, 1 pg, 100 fg, 10 fg, 1 fg and 100 ag per reaction and blank control (DW), respectively. The LoD of the m-LAMP assay for femA and mecA detection was 100 fg of genomic template per reaction

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