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Fig. 6 | Antimicrobial Resistance & Infection Control

Fig. 6

From: The rapid and visual detection of methicillin-susceptible and methicillin-resistant Staphylococcus aureus using multiplex loop-mediated isothermal amplification linked to a nanoparticle-based lateral flow biosensor

Fig. 6

Optimization of the amplification time for m-LAMP detection. Different amplification times (a, 20 min; b, 30 min; c, 40 min; d, 50 min) were tested at 63 °C. Biosensors 1, 2, 3, 4, 5, 6, 7, and 8 represent genomic DNA (methicillin-resistant S. aureus, ATCC43300) levels of 10 ng, 10 pg, 1 pg, 100 fg, 10 fg, 1 fg and 100 ag target template per reaction and negative control (DW), respectively. The best sensitivity was observed when the amplification lasted for 40 min (c)

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