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Table 1 The primers used in the present study

From: The rapid and visual detection of methicillin-susceptible and methicillin-resistant Staphylococcus aureus using multiplex loop-mediated isothermal amplification linked to a nanoparticle-based lateral flow biosensor

Primer name

Sequence and modifications

Length

Gene

F3

5′-GTCCTGAAAATAAAAAAGCACAT-3′

23 nt

femA

B3

5′-ACTTCCGGCAAAATGACG-3′

18 nt

 

FIP

5′-TGTTCTTCTTGTAGACGTTTACCTT-CGAGATAACTTACAACAACAACTTG-3′

50 mer

 

FIP*

5′-Dig- TGTTCTTCTTGTAGACGTTTACCTT-CGAGATAACTTACAACAACAACTTG −3′

50 mer

 

BIP

5′-ACCTATCTCTGCTGGTTTCTTCT-AATGCATTTGATGTACCACC-3′

43 nt

 

LF

5′-TCAATCTTTTGCTCATTTGC-3′

20 nt

 

LF*

5′-Biotin-TCAATCTTTTGCTCATTTGC-3′

20 nt

 

LB

5′-CCATTTGAAGTTGTTTATTATGC-3′

23 nt

 

F3

5′-GGCTCAGGTACTGCTATC-3′

18 nt

mecA

B3

5′-TTGTTATTTAACCCAATCATTGC-3′

23 nt

 

FIP

5′-ATGCCATACATAAATGGATAGACGT-CAAACAGGTGAATTATTAGCACTT-3′

49 nt

 

FIP*

5′-FAM-ATGCCATACATAAATGGATAGACGT-CAAACAGGTGAATTATTAGCACTT-3′

49 nt

 

BIP

5′-CCGAAGATAAAAAAGAACCTCTGCT-TTTTTGAGTTGAACCTGGTG-3′

45 nt

 

LF

5′-CATATGAAGGTGTGCTTAC-3′

19 nt

 

LF*

5′-Biotin-CATATGAAGGTGTGCTTAC-3′

19 nt

 

LB

5′-CAAGTTCCAGATTACAACTT-3′

20 nt

 
  1. Note: femA-FIP*, 5′-labeled with Dig when used in LAMP-LFB assay; femA-LF*, 5′-labeled with biotin when used in LAMP-LFB assay
  2. mecA-FIP*, 5′-labeled with FAM when used in the LAMP-LFB assay; mecA-LF*, 5′-labeled with biotin when used in the LAMP-LFB assay;
  3. Abbreviations: Dig digoxigenin, FAM carboxyfluorescein, nt nucleotide, mer monomeric unit