Table 1 The primers of genes used in this study
Target | Gene | Primer sequence (5′-3′) | Size of product (bp) | Reference |
|---|---|---|---|---|
femA | femA | F: TGCTATCCACCCTCAAACAGG R: AACGTTGTAACCACCCCAAGA | 450 | [13] |
mecA | mecA | F: TGCTATCCACCCTCAAACAGG R: AACGTTGTAACCACCCCAAGA | 286 | [17] |
SCCmec | ccrA2 | F: TAAAGGCATCAATGCACAAACACT R: AGCTCAAAAGCAAGCAATAGAAT | 937 | [18] |
ccrC | F: CCTTTATAGACTGGATTATTCAAAATAT R:CGTCTATTACAAGATGTTAAGGATAAT | 518 | Â | |
IS1272 | F: GCCACTCATAACATATGGAA R: CATCCGAGTGAAACCCAAA | 415 | ||
mecA-IS431 | F: TATACCAAACCCGACAACTAC R: CGGCTACAGTGATAACATCC | 359 | ||
MSCRAMMs genes | bbp | F: AACTACATCTAGTACTCAACAACAG R: ATGTGCTTGAATAACACCATCATCT | 575 | [5] |
cna | F: GTCAAGCAGTTATTAACACCAGAC R: AATCAGTAATTGCACTTTGTCCACTG | 423 | ||
eno | F: ACGTGCAGCAGCTGACT R: CAACAGCATYCTTCAGTACCTTC | 302 | ||
ebpS | F: CATCCAGAACCAATCGAAGAC R:CTTAACAGTTACATCATCATGTTTATCTTTG | 186 | ||
fnbA | F: GTGAAGTTTTAGAAGGTGGAAAGATTAG R: GCTCTTGTAAGACCATTTTTCTTCAC | 643 | ||
fnbB | F: GTAACAGCTAATGGTCGAATTGATACT R: CAAGTTCGATAGGAGTACTATGTTC | 524 | ||
fib | F: CTACAACTACAATTGCCGTCAACAG R: GCTCTTGTAAGACCATTTTCTTCAC | 404 | ||
clfA | F: ATTGGCGTGGCTTCAGTGCT R: CGTTTCTTCCGTAGTTGCATTTG | 292 | ||
Housekeeping genes | arc | up: TTGATTCACCAGCGCGTATTGTC dn: AGGTATCTGCTTCAATCAGCG | 456 | |
aro | up: ATCGGAAATCCTATTTCACATTC dn: GGTGTTGTATTAATAACGATATC | 456 | ||
glp | up: CTAGGAACTGCAATCTTAATCC dn: TGGTAAAATCGCATGTCCAATTC | 465 | ||
gmk | up: ATCGTTTTATCGGGACCATC | 429 | ||
dn: TCATTAACTACAACGTAATCGTA | Â | |||
pta | up: GTTAAAATCGTATTACCTGAAGG dn: GACCCTTTTGTTGAAAAGCTTAA | 474 | Â | |
tpi | up: TCGTTCATTCTGAACGTCGTGAA dn: TTTGCACCTTCTAACAATTGTAC | 402 | ||
ygi | up: CAGCATACAGGACACCTATTGGC dn: CGTTGAGGAATCGATACTGGAAC | 516 |