The prevalence and mechanism of triclosan resistance in Escherichia coli isolated from urine samples in Wenzhou, China

Table 1 Mutations of fabI and MICs of triclosan and antibiotics against E. coli strains

Isolates	Triclosan MICs (μg/mL)	Antibiotic MICs ^a (μg/mL)										Mutations in fabI ^b
Isolates	Triclosan MICs (μg/mL)	AMP	CIP	LVX	FEP	CAZ	ETP	IPM	GEN	NIT	TOB
DC8358	8	> 128^c	0.5	0.5	32	16	16	2	> 64	64	> 64	ND ^d
DC8419	4	> 128	> 32	64	16	16	< 0.5	< 0.25	> 64	32	32	Gly79Ala
DC8424	4	> 128	> 32	16	> 64	> 64	> 32	> 32	> 64	64	> 64	Ala69Thr
DC8603	2	> 128	> 32	> 64	64	32	< 0.5	< 0.25	< 2	> 128	< 2	ND
DC8724	8	> 128	> 32	32	32	64	16	1	> 64	16	> 64	ND
DC8361	0.125	< 4	< 0.25	0.5	< 1	< 2	< 0.5	< 0.25	< 2	16	< 2	Met2Arg; Val5Phe; Ala69Thr
DC8363	0.125	> 128	< 0.25	0.5	2	< 2	< 0.5	< 0.25	< 2	< 8	< 2	Ser5Leu; Gly79Ala
DC8400	0.125	> 128	< 0.25	< 0.25	< 1	< 2	< 0.5	< 0.25	< 2	16	< 2	Val4Ser; Ala69Thr
DC8413	0.25	> 128	< 0.25	< 0.25	< 1	< 2	< 0.5	< 0.25	< 2	< 8	< 2	Gly79Ala
DC8510	0.125	> 128	< 0.25	< 0.25	2	< 2	< 0.5	< 0.25	< 2	< 8	< 2	Gly79Ala; Asp 235Glu

^a MICs Minimum inhibitory concentration. AMP ampicillin, CIP ciprofloxacin, LVX levofloxacin, FEP cefepime, CAZ ceftazidime, ETP ertapenem, IPM imipenem, GEN gentamicin, NIT nitrofurantoin, TOB tobramycin. ^b Gly Glicine, Ala Alanine, Thr Threonine, Met Methionine, Arg Arginine, Val Valine, Phe Phenylalanine, Ser Serine, Leu Leucine, Asp aspartic acid, Glu glutamic acid. ^c The values in bold font indicate resistance. ^d ND Not detected

ISSN: 2047-2994