Skip to main content
Fig. 3 | Antimicrobial Resistance & Infection Control

Fig. 3

From: Simultaneous detection and ribotyping of Clostridioides difficile, and toxin gene detection directly on fecal samples

Fig. 3

Heat map and dendrogram based on ribosomal DNA fragment peak profiles of all fecal samples. Each column represents one sample. Numbers on the X-axis correspond to the reference ribotype assigned to the corresponding strain by conventional ribotyping by the Dutch National Reference Laboratory. Numbers on the Y-axis correspond to the DNA fragment length of the detected ribotype DNA fragment peaks/bands (in blue) and/or C. difficile toxin genes (red/orange) per sample. Toxin gene detection could not be performed in one sample due to insufficient DNA material (grey). ‘*’ = probable reference ribotype (by conventional ribotyping by the Reference Laboratory, band patterns of study strains were highly similar to patterns of reference strains except for a 1 or 2 bands difference); ‘?’ = unknown reference ribotype (by conventional ribotyping, an unknown band pattern was observed in the study strain that did not match the band pattern of one of the reference strains); ‘-’ = no reference ribotype (by conventional ribotyping, no band pattern was observed and therefore this study strain could not be matched to a reference ribotype); tcdA = C. difficile toxin A; tcdB = C. difficile toxin B; cdtA = C. difficile binary toxin A; cdtB = C. difficile binary toxin B, nc = nucleotides

Back to article page