Antibiogram and beta-lactamase genes among cefotaxime resistant E. coli from wastewater treatment plant

Table 3 Primer sequences, genes for beta-lactamase and the expected amplicon sizes

Group	Gene name	Primer sequence (5′–3′)	Expected amplicon size (bp)
ESBLS genes	*bla*_TEM	GTCGCCGCATACACTATTCTCA	258
	*bla*_TEM	CGCTCGTCGTTTGGTATGG	258
	*bla*_CTX-M	CGGGAGGCAGACTGGGTGT	381
	*bla*_CTX-M	TCGGCTCGGTACGGTCGA	381
	*bla*_SHV	GCCTTGACCGCTGGGAAAC	319
	*bla*_SHV	GGCGTATCCCGCAGATAAAT	319
Carbapenemase genes	*bla*_VIM	GATGGTGTTTGGTCGCATA	390
	*bla*_VIM	CGAATGCGCAGC	390
	*bla*_OXA-1	TTCTGTTGTTTGGGTTTCGC	190
	*bla*_OXA-1	ACGCAGGAATTGAATTTGTTC	190
	*bla*_KPC-2	GCTTCCCACTGTGCAGCTCATTC	213
	*bla*_KPC-2	CGCCCAACTCCTCAGCAACAATTG	213
	*bla*_NDM-1	GGTGCATGCCCGGTGAAATC	660
	*bla*_NDM-1	ATGCTGGCCTTGGGGAACG	660

ISSN: 2047-2994