Table 1 Genes and their primers sequences for molecular characterization of K. pneumoniae
Target genes | Primer sequence | Ampilicon size (Bp) | References |
|---|---|---|---|
khe | F: TGATTGCATTCGCCACTGG R: GGTCAACCCAACGATCCTG | 428 | Neuberger et al. [53] |
WzyK1 | F: GGTGCTCTTTACATCATTGC R: GCAATGGCCATTTGCGTTAG | 1283 | Turton et al. [54] |
WzyK2 | F: GACCCGATATTCATACTTGACAGAG R: CCTGAAGTAAAATCGTAAATAGATGGC | 641 | Turton et al. [54] |
WzxK5 | F: TGGTAGTGATGCTCGCGA R: CCTGAACCCACCCCAATC | 280 | Turton et al. [54] |
WzyK20 | F: CGGTGCTACAGTGCATCATT R: GTTATACGATGCTCAGTCGC | 741 | Fang et al. [9] |
WzxK54 | F: CATTAGCTCAGTGGTTGGCT R: GCTTGACAAACACCATAGCAG | 881 | Fang et al. [9] |
Wzy57 | F: CTCAGGGCTAGAAGTGTCAT R: CACTAACCCAGAAAGTCGAG | 1037 | Pan et al. [41] |
WzyK3 | F: TAGGCAATTGACTTTAGGTG R: AGTGAATCAGCCTTCACCT | 549 | Fevre et al. [10] |
BlaKPC | F-ATG TCA CTG TAT CGC CGT CT R-TTT TCA GAG CCT TAC TGC CC | 538 | Dallenne et al. [19] |
BlaIMP-1 | F-TGA GCA AGT TAT CTG TAT TC R-TTA GTT GCT TGG TTT TGA TG | 139 | Dallenne et al. [19] |
BlaIMP-2 | F-GGC AGT CGC CCT AAA ACA AA R-TAG TTA CTT GGC TGT GAT GG | 139 | Dallenne et al. [19] |
BlaVIM | F-GAT GGT GTT TGG TCG CAT A R-CGA ATG CGC AGC ACC AG | 390 | Dallenne et al. [19] |
BlaNDM | F-GGT TTG GCG ATC TGG TTT TC R-CGG AAT GGC TCA TCA CGA TC | 521 | Mushi et al. [24] |
BlaOXA-48 like | F-TTG GTG GCA TCG ATT ATC GG R- GAG CAC TTC TTT TGT GAT GGC | 281 | Dallenne et al. [19] |