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Table 1 Primers used in this study

From: Characterisation of mobile colistin resistance genes (mcr-3 and mcr-5) in river and storm water in regions of the Western Cape of South Africa

Target gene Primer name DNA sequence (5′ → 3′) Amplicon size (bp) Reference
mcr-1 CLR5-F 5′-CGGTCAGTCCGTTTGTTC-3′ 309 [1]
CLR5-R 5′-CTTGGTCGGTCTGTAGGG-3′
MCR-1YF 5′-CATATGATGATGCAGCATACTTCT GTGTGGTACCG-3′ 1632 This study
MCR-1YR 5′-CTCGAGTCAGCGGATGAATGCGGT-3′
mcr-2 MCR2-IF 5′-TGTTGCTTGTGCCGATTGGA-3′ 567 [2]
MCR2-IR 5′-AGATGGTATTGTTGGTTGCTG-3′
mcr-3 MCR3-F 5′-TTGGCACTGTATTTTGCATTT-3′ 542 [3]
MCR3-R 5′-TTAACGAAATTGGCTGGAACA-3′
MCR-3YF 5′-ATGCCTTCCCTTATAAAAATAAA-3′ 1622 This study
MCR3-YR2 5′-TCAATTATTCCGACATTGCTTA-3′
MCR-3YF2 5′-TGAAAGGGGATAAGCTGGTT-3′ Not applicable This study
MCR-3YR3 5′-CATAATCTTGATAGTATAGTGCTG-3′
MCR-3 YF3 5′-CATATGATGCCTTCCCTTATAAAA ATAAAAATTGT-3′ 1622 This study
MCR-3 YR4 5′-CTCGAGTCAATTATTCCGACATTGC-3′
mcr-4 MCR-4 FW 5′-ATTGGGATAGTCGCCTTTTT-3′ 487 [36]
MCR-4 RV 5′-TTACAGCCAGAATCATTATCA-3′
mcr-5 MCR5_FW 5′-ATGCGGTTGTCTGCATTTATC-3′ 1644 [5]
MCR5_REV 5′-TCATTGTGGTTGTCCTTTTCTG-3′
mcr-6 MCR-6F 5′-GTCCGGTCAATCCCTATCTGT-3′ 556 [8]
MCR-6R 5′-ATCACGGGATTGACATAGCTAC-3′
mcr-7 MCR-7F 5′-TGCTCAAGCCCTTCTTTTCGT-3′ 892
MCR-7R 5′-TTCATCTGCGCCACCTCGT-3′
mcr-8 MCR-8F 5′-AACCGCCAGAGCACAGAATT-3′ 667
MCR-8R 5′-TTCCCCCAGCGATTCTCCAT-3′
mcr-9 MCR-9YF 5′-ATGCCTGTACTTTTCAGGGTGAAAG-3′ 1619 This study
MCR-9YR 5′-TTCCGCGAATGCCGTGGCTAA-3′