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Table 2 Colistin resistant isolates and mcr-3 and mcr-5.1 colistin resistance genes detected in river and storm water in the Western Cape of South Africa during 2019–2020

From: Characterisation of mobile colistin resistance genes (mcr-3 and mcr-5) in river and storm water in regions of the Western Cape of South Africa

Sampling sites Plankenburg river Eerste river Berg river Muizenberg storm water Fish Hoek storm water
P1 P2a P2b E1 E2 B1 B2 B3 M FH
Sampling date May 2019 May 2019 Jan 2020 Jan 2020 Jan 2020 Jan 2020 Jan 2020 Jan 2020 Nov 2019 Nov 2019
Proportion of phenotypically colistin resistant colonies, n (%) 496/620
(80%)
5670/13000
(44%)
7900/42000
(19%)
400/1050 (38%) 85,000/188000 (45%) 840/1350
(62%)
14,700/186000 (7.9%) 540/1480 (36%) 320/6400 (5%) 170/940 (18%)
Colistin resistant isolates (n, MIC) Aeromonas jandei (3, MIC: 32),
Aeromonas sobria (3, MIC: 32),
Aeromonas hydrophila (2, MIC: 64),
Pectobacterium carotovorum (1, MIC: 16)
Raoultella ornithinolytica (10, MIC: 64),
Franconibacter helveticus (2, MIC: 64),
Kluyvera ascorbata (2, MIC:16),
Citrobacter freundi (1, MIC:16),
Klebsiella oxytoca (1, MIC:16),
Pantoea agglomerans (1, MIC:16),
Pluralibacter gergoviae (1, MIC:16))
Aeromonas hydrophila (11, MIC: 64),
Salmonella enterica (1, MIC: 64)
Aeromonas hydrophila (2, MIC: 64) Aeromonas hydrophila (11, MIC: 32 or 64),
Klebsiella oxytoca (1, MIC:4)
Aeromonas jandei (5, MIC: 64),
Raoultella ornithinolytica (4, MIC: 4 or 64),
Aeromonas hydrophila (3, MIC: 32 or 64),
Klebsiella oxytoca (1, MIC: 8),
Pectobacterium carotovorum (1, MIC: 16)
Aeromonas hydrophila (3, MIC: 64) Aeromonas hydrophila (5, MIC: 64),
Aeromonas veronii (4, MIC: 4 or 8),
Aeromonas sobria (3, MIC: 4),
Aeromonas jandei (1, MIC: 4)
Aeromonas hydrophila (13, MIC: 32 or 64) Aeromonas hydrophila (1, MIC: 64),
Enterobacter kobei (1, MIC: 64)
Number of isolates of each species in which mcr genes were detected (n) None
detected
None
detected
None detected None detected None
detected
A. jandei
mcr-3.33 (1)
mcr-3.34 (1)
mcr-3.35 (1)
mcr-3.36 (2)
None detected A. veronii
mcr-3.37 (1)
None detected None detected
mcr genes detected directly from the water sample (eDNA) None
detected
mcr-3
mcr-5.1
mcr-3
mcr-5.1
None detected mcr-3
mcr-5.1
mcr-3 None detected mcr-3
mcr-5.1
mcr-3
mcr-5.1
mcr-5.1