Environmental surveillance of ESBL and carbapenemase-producing gram-negative bacteria in a Ghanaian Tertiary Hospital

Acolatse, Joseph Elikem Efui; Portal, Edward A. R.; Boostrom, Ian; Akafity, George; Dakroah, Mavis Puopelle; Chalker, Victoria J.; Sands, Kirsty; Spiller, Owen B.

doi:10.1186/s13756-022-01090-2

Antimicrobial Resistance & Infection Control

Table 3 A summary of each isolate with whole genome sequencing (WGS) data

From: Environmental surveillance of ESBL and carbapenemase-producing gram-negative bacteria in a Ghanaian Tertiary Hospital

Isolate code, location in ward and bacterial species Identification (ID)			Antibiotic phenotypic data MIC, ug/ml					Carbapenemase ARG			Number of antibiotic resistance genes per class										Virulence factors
Isolate code	Location	ID	MER	COL	CEF	TIG	GEN	NDM	OXA-48	Other CARB ARG	AMG	BLA	RIF	TRI	PHE	FOS	MAC	FLU	SUL	TET	VF	BAVF
NICU0001	Cot handle	ACI	32	0.125	128	0.5	16	Y	N	blaOXA-23, blaOXA-67	2	3	0	0	0	0	0	0	1	1	ND	ND
NICU0008	Bed wheels	PSS	64	1	128	1	0.5	N	N	blaDIM	0	0	1	0	0	0	0	0	0	0	ND	ND
OG0001	Bed handle	ACB	256	1	> 256	0.5	256	Y	N	blaOXA-23, blaOXA-67	1	4	0	0	0	0	2	0	1	2	37	5
OG0003	Mattress	ACB	256	1	> 256	2	256	Y	N	blaOXA-23	4	5	0	1	0	0	2	0	2	1	36	4
OG0004	Pillow	ACB	128	1	> 256	0.5	128	Y	N	blaOXA-23, blaOXA-67	1	4	0	0	0	0	2	0	1	2	32	5
OG0008	Bedside cabinet	ACB	64	1	> 256	2	128	Y	N	blaOXA-23, blaOXA-699	1	4	0	0	0	0	2	0	1	1	33	4
OG0018	Sphygmomanometer	ACB	256	1	> 256	0.5	256	Y	N	blaOXA-23, blaOXA-67	1	4	0	0	0	0	2	0	1	2	35	5
OG0020	Desk surface	ACB	256	1	> 256	0.5	256	Y	N	blaOXA-23, blaOXA-67	1	4	0	0	0	0	2	0	1	2	34	5
OG0025	Desk surface	ACB	128	1	> 256	0.5	256	Y	N	blaOXA-23, blaOXA-67	1	4	0	0	0	0	2	0	1	2	33	5
OG0026	Washroom door handle	ACB	256	1	> 256	1	256	Y	N	blaOXA-23, blaOXA-67	1	4	0	0	0	0	2	0	1	2	35	5
OG0027	Tap handle	ACB	256	1	> 256	1	256	Y	N	blaOXA-23, blaOXA-67	1	4	0	0	0	0	2	0	1	2	37	5
OG0030	Bedsheet	ACB	128	1	> 256	0.5	256	Y	N	blaOXA-23, blaOXA-67	1	4	0	0	0	0	2	0	1	2	33	5
OG0030.2	Bedsheet	ACB	256	1	> 256	0.5	128	Y	N	blaOXA-23, blaOXA-67	1	4	0	0	0	0	2	0	1	2	33	5
OG0032	Wall socket switch	ACB	256	1	> 256	0.5	> 256	Y	N	blaOXA-67	1	3	0	0	0	0	2	0	1	2	31	5
PW0001	Bed handle	ACB	128	1	> 256	1	128	Y	N	blaOXA-90	3	4	0	1	0	0	2	0	1	1	33	4
PW0006	Mattress	ACI	64	0.06	256	0.5	16	Y	N	blaOXA-420	4	2	0	1	1	0	2	0	1	0	ND	ND
PW0007	Pillow	PSS	ND	ND	ND	ND	ND	N	N	blaVIM-5	1	3	0	1	0	0	2	1	0	0	ND	ND
PW0008	Pulse oximeter	ACN	ND	ND	ND	ND	ND	Y	N	blaOXA-23	1	2	0	0	0	0	2	0	1	1	6	2
PW0012.1	Bedside cabinet	ACB	64	0.5	> 256	8	256	Y	N	ND	3	4	1	0	1	0	2	0	2	1	31	3
PW0012.2	Bedside cabinet	KPN	128	32	128	2	64	Y	Y	ND	3	5	0	2	0	1	2	3	2	1	49	0
PW0019	Tap handle	ACZ	256	2	> 256	4	> 256	Y	N	blaOXA-356	5	4	0	0	1	0	2	0	1	2	ND	ND
PW0055	IV stand	ACT	64	0.5	> 256	0.5	128	Y	N	blaOXA-58	3	2	0	0	1	0	2	0	1	1	ND	ND
PW0056	Bed sheet	ACJ	64	8	> 256	1	> 256	Y	N	blaOXA-58	4	2	0	0	1	0	2	0	1	1	ND	ND
PW0057	Thermometer	ACN	128	0.5	> 256	0.5	128	Y	N	blaOXA-23	1	2	0	0	0	0	2	0	1	1	6	2
PW0063	Computer keyboard	ACN	128	1	> 256	0.5	128	Y	N	blaOXA-23	1	2	0	0	0	0	2	0	1	1	5	2
PW0064	Washroom door handle	ACI	32	0.125	256	0.25	8	Y	N	blaOXA-420	5	2	0	1	0	0	2	0	1	0	ND	ND
PW0068	Bed wheels	ACS	32	0.125	> 256	0.25	0.5	Y	N	blaOXA-235, blaOXA-58	1	3	1	0	0	0	2	0	1	1	ND	ND
PW0072	Pillow	ACO	16	0.125	256	0.5	64	Y	N	blaOXA-58, blaOXA651	4	4	1	0	1	0	1	0	1	1	ND	ND

The isolate code, location species, carbapebemase ARG, antibiotic phenotypic data, number of ARG per antibiotic class, total virulence factor genes (VF) and biofilm associated virulence factors (BAVF) are listed
^*Species: ACI, Acinetobacter indicus; PSS, Pseudomonas stutzeri; ACB, Acinetobacter baumannii; ACN, Acinetobacter nosocomialis; KPN, Klebsiella pneumoniae; ACZ, Acinetobacter berenziniae; ACT, Acinetobacter towneri; ACJ, Acinetobacter junii; ACS, Acinetobacter sp.; ACO, Acinetobacter johnsonii
Antibiotic phenotypic data: MER, meropenem; COL, colistin; CEF, cefotaxime; TIG, tigecycline; GEN, gentamicin
Antibiotic resistance genes: AMG, aminoglycoside; BLA, β-lactam; RIF, rifampicin; TRI, trimethoprim; PHE, phenicol; FOS, fosfomycin; MAC, macrolide; FLU, fluoroquinolone; SUL, sulphonamide; TET, tetracycline

Back to article page

ISSN: 2047-2994

Contact us

General enquiries: journalsubmissions@springernature.com