Environmental surveillance of ESBL and carbapenemase-producing gram-negative bacteria in a Ghanaian Tertiary Hospital

Table 4 De novo assembly metrics for the four isolates with supplementary long-read sequencing

Isolate code	Species identification	No'contigs	Chromosome, complete	Size of plasmid with carbapenemase ARG(s)	ARGs on plasmid	Plasmid type/BLAST similarity to
PW0012.1	Acinetobacter baumannii	7	3,820,549 bp, Y	309,568 bp, Y	ARR-3, sul1, bla_NDM-1, aph(3′)-Via, bla_CARB-16, aac(3)-Iid, aph(3″)-Ib, aph(6)-Id, mph(E), msr(E), tet(X3), floR, sul2	NZ_CP042365.1
PW0012.2	Klebsiella pneumoniae	8	5,300,304 bp, Y	273,549 bp, Y	bla_NDM-1, bla_OXA-48, aph(3')-VI, mph(E), msr(E), armA, sul1, dfrA12	IncHI1B/IncFIB, NZ_CP050156.1
PW0068	Acinetobacter sp. (30% unclassified reads)	12	2,799,419 bp, Y	53,570 bp, Y	tet(39), mph(E), msr(E), bla_NDM-1, aph(3')-VI, bla_OXA-58, sul1	NZ_LC591943.1
OG0025	Acinetobacter baumannii	26	3,657,799 bp, Y	268,855 bp, N	bla_NDM-1, sul2, bla_OXA-23, msr(E), mph(E), tet(39), aac(3)-Iid	NZ_MK134375.1

The number of contigs, the size of bacterial chromosome and plasmid with antibiotic resistance genes (ARG) of interest are shown (Y, indicates the chromosome/plasmid was circularised/complete; N, indicates the contig is linear). All ARG on the target plasmid are listed and the plasmid inc type is shown were applicable. The most similar plasmid as per mash dist analysis in PLSDB is listed

ISSN: 2047-2994