Late breaking abstracts from the 6th international conference on prevention & infection control (ICPIC 2021)

Background: COVID-19 outbreaks with high mortality rates have been reported from long-term care facilities (LTCFs) worldwide despite infection control and prevention (ICP) measures. However, no systematic data exist on COVID-19 burden and its risk factors in Swiss-LTCFs. Aims: to assess (i) COVID-19 incidence and-related mortality for the year 2020 and their influencing factors, (ii) vaccination rates of residents and health care workers (HCW). Methods: LTCFs from cantons of St Gallen (SG) and Vaud (VD) (Eastern and Western Switzerland) were invited to participate in this cohort study. In May 2021, we collected COVID-19 cases and-related deaths 2020 and their potential risk factors at institutional level (e.g. size, number of single beds, staffing, ICP measures, aggregated resident characteristics) as well as vaccination rates of residents and HCW. Risk factor analysis and prospective surveillance of COVID-19 is ongoing. Results: We enrolled a total of 59 institutions (33 from SG, 26 from VD), with a median of 46 (IQR 33.69) occupied beds. In 2020, median COVID-19 incidence was 38.6 per 100 occupied beds, with higher rates in VD than in SG (48% vs. 29.4%, p = 0.028) (Fig. 1). Rates varied widely among LTCFs, with some institutions reporting no COVID-19 cases. On average (median), one-fifth (20%) of COVID-19 cases died and COVID-19 related deaths accounted for 21.6% of total deaths. Regarding ICP measures, 54/59 institutions (91.5%) recommended using gowns in contact with COVID-19 patients, whilst use of other PPE (i.e. FFP2 masks, glows and googles) was more heterogeneous. Only a few LTCFs reported having ever used regular testing of asymptomatic residents (6/59, 10.2%) or HCW (14/59, 23.7%) as a prevention strategy. Nearly half of them (27/59, 45.8%) never banned visits or only in outbreak situations. Of 2786 residents, a median of 72.2% per facility received ≥1 dose of COVID-19 vaccine. COVID-19 vaccine uptake among HCW was low (28.6%) (Fig. 1). Conclusion: COVID-19 burden was highly heterogeneous in Swiss LTCFs. Further analyses will reveal factors, which could potentially explain these differences. Of note, we found a relatively high COVID-19 vaccine uptake among care home residents, whilst vaccine coverage of HCW is low.


O46 Out of a Comfort Zone: Challenges of Infection Prevention in a 4000 Bedded Intensive Care Unit Built in an Exhibition
Introduction: The 2019-2021 SARS-2 Pandemic has created a number of challenges for healthcare systems globally. In March 2020, the UK NHS looked about to be overwhelmed with the number of patients requiring hospitalisation and intensive care management. The decision was made to create a number of temporary facilities called 'Nightingale Hospitals' in exhibition and conference centres around the UK. The London facility was designed as a 4000 bedded Intensive Care Unit (ICU). This paper describes the challenges in designing, building and running an ICU in an exhibition centre. Objectives: To describe the design process for a hospital in an exhibition centre. To discuss the challenges experienced during the building process. To describe the challenges faced with staffing such a hospital. To discuss areas in which risk assessments had to be made and practices modified to mitigate suboptimal conditions. Methods: NHS Designers and Military planners worked in conjunction with the Infection Prevention and Control Team (IPCT) to work with what was available. The whole of the clinical area was deemed to be an Aerosol-generating Procedure (AGP) zone. No oxygen supplies or hot water, minimal access to cold water, almost no drainage and a lack of physical spaces in which to carry out many of the necessary procedures were amongst the challenges faced and were overcome either by design or by changes to optimal practices through mitigation measures. The IPCT had key role to play in ensuring staff and patient safety, including training, stock management of Personal Protective Equipment (PPE) and donning and doffing procedures. Results: The London Nightingale Hospital became a fully functioning ICU within 10 days of the build commencing and the first patients were admitted within a few days. Thankfully it was only sparingly used as the national lockdown had an effect. In all, 72 patients were admitted with a survival rate of 63%, which was comparable with other established ICUs in London. Transmission rates of COVID-19 in staff were very low in those working clinically. The hospital was closed in June 2020 but reopened in January 2021 in a different guise (rehabilitation) with a smaller number of beds (300) but better facilities as a result of the learning from the first iteration. Conclusion: A temporary hospital built in an exhibition centre successfully managed a number of ICU patients. Lessons learned will shape future planning should this type of facility be required again.

Disclosure of interest
None declared. Introduction: The rapid effectiveness of alcohol-based hand rub(ABHR) on microbial pathogens, including viral variants, inspired unprecedented demand for ABHR during the COVID-19 pandemic. A wide variety of new ABHR products that were rapidly produced and released to the public appear to have introduced new and unacceptable human safety risks. Objectives: While the long-term public health impact of exposure to adulterated ABHR will require further study, the immediate need to assess the safety and efficacy of ABHR motivated this study. Methods: Sampling of ABHR provided to the public in community settings (e.g., by a medical center) occurred across a wide variety of global community settings. When possible, ABHR samples of 30-50 ml were analyzed for: alcohol %, visible impurities and chemical impurities (i.e., acetal, acetaldehyde, benzene, and methanol). Validated analytical methods (good laboratory practices (GLP) or International Organization for Standardization (ISO) standards) were used for quantitative measurements. Photographs were taken for visual assessment of packaging design + labeling risks. Results: Alcohol concentrations less than a typical minimum antimicrobial efficacy level of 60% were measured in ≥ 7% of the ABHR samples. Unknown visible impurities were observed in ≥ 11% of samples. Levels of regulated impurities (acetal, acetaldehyde, benzene, and methanol) in excess of US FDA Guidance were observed in ≥ 43% of samples. Photographs demonstrate numerous risks associated with package design and open refillable ("bulk") dispensers, including no lot code nor expiry, and lack of traceable labeling. Conclusion: During the COVID-19 Emergency, demand for ABHR prompted new supply sources, followed by inscrutable donations of surplus products. Though unintended, allowing tainted and poor quality ABHR into communities places clinicians and the public at unnecessary risk. Guidance to consumers, businesses, and communities including medical centers, nursing homes, schools, offices, and retail settings, on the proper evaluation, use, and handling of ABHR, and safe disposal of adulterated or suspect product is needed. Regulatory guidance and enforcement addressing dangerous practices evident in this study including refilling of pump bottles/dispensers with a variety of ABHRs is needed immediately to reduce future exposure to unsafe, ineffective, mislabeled or unlabeled ABHR products. Disclosure of interest J. Arbogast Employee of: GOJO Industries (hygiene products manufacturer), K. Kucera Employee of: Valisure LLC, D. Light Employee of: Valisure LLC, D. Yeomans Employee of: GOJO Industries (hygiene products manufacturer), C. Manuel Employee of: GOJO Industries (hygiene products manufacturer).

Introduction:
The guideline-driven and widely implemented single room isolation strategy for respiratory viral infections (RVI) such as influenza or respiratory syncytial virus (RSV) can lead to a shortage of hospital beds. Alternative strategies to prevent hospital-acquired respiratory viral infections (HARVI) are needed. Objectives: Based on promising results of a pilot study using droplet precautions on-site (DroPS) during the influenza season 2018/19, this strategy was implemented in multiple hospital wards. We assessed the "real-life" safety of DroPS by measuring the rate of HARVI after its implementation. Methods: Prospective observational study during the influenza season 2019/20 at a tertiary and secondary referral hospital. The study was prematurely stopped at the beginning of the COVID-19 pandemic (17th March 2020). DroPS was used for patients hospitalised with suspected or proven RVI. Hospitalised patients with no respiratory isolation in the two days following admission were considered "at risk" for the acquisition of HARVI. They were screened daily for the onset of new respiratory symptoms. Once an "at risk" patient developed symptoms, an influenza/RSV molecular rapid test was performed. The two main outcomes were the rate of clinical and laboratory-confirmed HARVI (influenza or RSV). Results: We included 1′996 hospitalisations with a total of 8′955 "at risk" hospital days for HARVI. Median age was 71 years . HARVI was clinically diagnosed in 11/1′996 (0.6%) hospitalisations. All patients with clinical diagnosis were microbiologically screened and three confirmed (0.15%; 2 × RSV, 1 × influenza B). Conclusion: Droplet precautions on site (DroPS) may represent a safe, simple and resource-saving alternative to the traditional pathogenbased single room strategy for RVI in non-pandemic circumstances.

Disclosure of interest
None declared. Introduction: Vaccination of Healthcare workers (HCWs) is a crucial element of the strategy against COVID-19 to conduct an effective immunization campaign among general population. Objectives: A cross-sectional survey was conducted to assess attitudes, sources of information and practices among HCWs in relation to COVID-19 vaccination. Methods: From February 19th to April 23rd 2021 an anonymous voluntary questionnaire was sent to the mailing list of SIMPIOS and to the main National Health Service structures. Data collected through the Survey-Monkey platform was subjected to univariate and multivariate analysis to identify factors significantly and independently associated with the variables of interest. Results: 2137 HCWs answered. Hesitancy is greater in females, in those with lower concern about COVID-19, in nurses, auxiliary nurses (AN) and healthcare assistants. The most hesitant professionals are more likely not to recommend vaccination to either their patients or their relatives, while concern about COVID-19 increases the practice of recommending vaccination to family members, but not to patients. HCWs are mostly in favor of mandatory vaccination (61.22%). Female sex, a lower education level, greater hesitancy and refusal to adhere to flu vaccination campaigns are factors influencing the aversion to mandatory vaccination. Institutional source of information (eg. World Health Organization) are the most used by all categories. Scientific literature is more used by professionals working in the northern regions of Italy and by those dealing with infection control and hospital hygiene, infectious diseases, emergencies and critical area. HCWs working in south-central regions, nurses, AN, healthcare technicians, administrators and HCWs with a lower education level are more likely to choose internet, television, newspapers, family and friends' advice as sources of information. Conclusion: Communication in support of COVID-19 immunization campaigns should consider the differences between the various HCWs professional categories, to reach all professionals in a homogeneous way, involving even the most hesitant ones.

Disclosure of interest
None declared. Introduction: Regular rounds by Infection control team is important to convince the staff that the principles of infection control in the ICU are based on simple concepts of surveillance and these are the basic measures that are easy to implement at the bedside to prevent hospital acquired infections. Objectives: This study will evaluate the impact of daily infection control rounds in Intensive Care Unit (ICU) of a newly set up tertiary oncology centre. Methods: This is a prospective observational study conducted in the Surgical-ICU of MPMMCC Varanasi, India. This is 350 bedded tertiaryoncology-centres,fully commissioned on January-2021.Checklist for daily-ICU-rounds was prepared by using CDC guidelines and daily round was taken by Infection control nurse. Following strategies were considered in the checklist; biomedical waste, Personal-protectiveequipment measure, Environmental-cleaning, hand-hygiene-audit and Device-maintenance-checklist. All variables were analysed to check the compliance or non-compliance of each strategy in the ICU. Results: 30% observations of BMW were noncompliant (NC). Most common was mixing of BMW (12%) followed by improper disposal (10%) and overfilling of carts (8%). Sharp disposal was improper in one-third observations mainly due to wrong disposal (60%) followed by uncovered-sharp (30%). PPE measures were followed in 99.3% times. NC was mainly seen in mainly doctors (6.5%). Adherence to environmental-cleaning seen in only 47% observations which was mainly due to NC activity seen in preparation of fresh-hypochloritesolution. Adherence to device maintenancedone in 67% patients. There is decrease-rate of CLABSI and CAUTI in ICU (CLBASI Rate:9.01 to 0.00, CAUTI rate (4.78-0.)/1000 patient days. Maximum hand hygiene compliance was observed among nursing officer (60.4%) followed by Technician (41%) and Doctors (26%). Maximum attempt of handhygiene were done before and after touching patients seen in nurses (46%, 50%),followed by technician (42%, 33%) and doctors (33%, 33%). Conclusion: BMW,environmental cleaning, hand hygiene activity are essential pillar of strengthening of ICP in ICU. This study strongly confirm that prevention of HAI in ICU can be effective by daily round by Infection control team.

Disclosure of interest
None declared. Introduction: Otomycosis is an infection of the external auditory canal.The fungal agents that colonize this canal can lead to complications.The knowledge of auricular fungal carriage could be a good indicator for mycological surveillance. Objectives: The objective of this study was to determine the frequency of fungal strains colonizing the external auditory canal in patients at the University Hospital of Angré.

Methods:
This cross-sectional study was carried out among patients who came to Ear, Nose and Throat clinic at the University Hospital of Angré from February to June 2021. For each patient, ear sample was taken using sterile swabs and transferred to the Parasitology-Mycology Unit for mycological analysis.Direct examination and culture on Sabouraud-Chloramphenicol medium were performed for each sample. The identification and antifungal sensitivity of strains were done using the VITEK 2 Compact ® . Results: Of 140 samples, 19(13.8%) yielded positive cultures 13.8%.Candida parapsilosis was the most isolated yeast species (57.0℅) while Aspergillus flavus (21.0%) was the common mold identified.C. albicans and C. parapsilosis were sensitive to antifungals tested while C. famata and C. tropicalis were resistant to caspofungin and micafungin. Fungal species identified was correlated with bathing (p = 0.025) and ear pruritus (p = 0.023). Conclusion: Fungal colonization of external auditory canal revealed various species with a predominance of C. Parapsilosis. An emergence of yeast strains resistant to antifungal was observed.Mycological surveillance of fungi isolated from ear should be managed in further studies using phenotypic and genotypic profiles. Keywords:External auditory canal-Colonization -Fungi -Antifungal Disclosure of interest None declared. Introduction: Singapore has seen the emergence of Candida auris as an emerging multi-resistant fungal infection while other MDROs are endemic here. Objectives: A clinical audit was conducted to measure the efforts to prevent and control transmission of C auris. Methods: A retrospective audit of all patients in the hospital epidemiology database diagnosed with C auris from April 2019 to June 2021 was conducted. Prior to Sept 2020, screening for C.auris was done using swabs of nares, axilla and groin of all patients coming from overseas/with recent overseas hospital stay, and contact tracing of newly diagnosed C auris patients. Screening swabs were cultured by standard microbiological methods (CLSI). After Sept 2020, all patients infected/colonized with carbapenem resistant Enterobacteriaceae (CRE) were also screened for C. auris. Methicillin resistant Staphylococcus aureus (MRSA) screening is routinely done at admission, ward transfer and before certain surgeries while CRE screening is done using a questionnaire. Data was analysed using Microsoft Excel. Results: Ten patients were diagnosed with C auris infection/colonization from April 2019 to June 2021. Two of 74 (2.7%) were found from screening of CRE patients and none from overseas screening. An additional case screened positive from contact tracing. Nine of the C. auris isolates were Clade I, the last one was not from any known clade. Nine C auris patients were in single rooms at detection and only one from open ward. In contrast, over the last month, 28 nosocomial MRSA acquisitions identified were all from open wards and none from single rooms. Conclusion: We found that 2.7% of patients infected/colonized with CRE were also colonized with Candida auris. Unlike new MRSA colonization, new C. auris cases occurred commonly in single rooms rather than open wards (p < 0.0001). While C. auris has some similarities with other MDROs which can guide screening strategies, there are clearly differences that should be explored in large multi-center prospective studies.

Disclosure of interest
None declared. .32 ± 6.34 μg/ ml), but low efficacy -against P.aeruginosa (MBC = 106.98 ± 9.28 μg/ml). CHX, OCT had a similar trend. CHX demonstrated more than twice lower activity against S.aureus, E.coli, K.pneumonia, A.baumannii than DCM (p < 0.001). P.aeruginosa were found to be less susceptible to DCM, CHX, OCT (p < 0.001). The MBC values of OCT for A.baumannii strains were 28.23 ± 3.73 μg/ml. PHMB demonstrated twice higher bactericidal properties against P.aeruginosa (52.08 ± 6.56 μg/ml) comparably to other antiseptics (p < 0.001), was alike effective for E.coli, K.pneumonia, but showed twice lower activity against A.baumannii. Poor anti-staphylococcal activity was found in PHMB (MBC = 179.69 ± 59.57 μg/ml). Conclusion: Reference and clinical strains of pathogens of the first and second priority categories by the WHO demonstrate high susceptibility to QAC antiseptics, with predominance of DCM activity against S.aureus, E.coli, K.pneumonia, A.baumannii and PHMB against P.aeruginosa (p < 0.001).

Disclosure of interest
None declared. Objectives: The objective of our study was to investigate the perception and experiences of patients on preoperative managament in patients participating in a trial implementing universal decolonization with polihexanide. Methods: Patients scheduled for hip or knee joint arthroplasty in 5 participating orthopedic centers were included between January 2017 and December 2018. Patients that received decolonization sets containing polihexanide and consented to participate in the study were asked to answer a questionnaire consisting of 14 closed items with a 5 point Likert scale. Patients received the questionnaire on the day of their preoperative visit and were asked to return it in an envelope to nursing staff upon admission. Results: Of 1866 patients that consented to participate in the study 1381 (74.0%) returned the questionnaire. Of those 1333 patients (96.5%) answered that they were at least in part compliant with the protocol and used the set. 1221 of the patient felt that they were sufficiently informed about application and potential adverse effects. 1224 patients used the decolonization set according to manufacturer's recommendations. 239 patients were not able to use the set without help. 251 patients had mild to moderate adverse events. Dryness of skin and flavor of oral solution were most frequently noted. Additional factor on the patients experience were investigated. Conclusion: Patients felt well informed about potential risk and general complications prior to their surgery. They are willing to perform additional task in preparation. Implementation of universal decolonization created a low number of adverse events.  -52397). The SARS-CoV-2 nucleocapsid protein (NR55344, Avi-Histag, Biotin-Labeled, BEI Resources, NIAID, ATCC) was also tested for susceptibility to damage. aPDT was carried out by exposing each viral component to a photosensitizer formulation containing 320 uM methylene blue in an aqueous adjuvant, immediately followed by 36 J/cm 2 (120 s) of non-thermal laser light at 664 nm (Steriwave ® aPDT system, Ondine Biomedical Inc., Vancouver, B.C.). Illumination was conducted in a custom thin-film cell replicating the anterior nasal architecture. Control solutions were identically treated but without light exposure. 50 ul samples of treated and control solutions were evaluated by SDS-PAGE using SYPRO Ruby Protein Gel Stain.

P079c Patients Perception and Experience on Universal Decolonization with Polihexanide
Results: SDS-PAGE evaluation of all treated RBD, spike and SARS-CoV-2 nucleocapsid protein samples showed no detectable protein remaining after 2 min exposure to photodynamic disinfection treatment. All no-light control samples remained unaffected. Conclusion: A 2-min photodynamic disinfection procedure was demonstrated to destroy RBD's, spike protein and nucleocapsid protein of major SARS-CoV-2 viral variants in circulation, expanding on previous work evaluating RNA damage in this virus. This outcome supports the use of aPDT as a potential SARS-CoV-2 suppression technique. Results: Twelve HCWs (12/90, 13.3%) were infected with COVID-19 during September 2020, all were symptomatic, none hospitalized. Source of infection was categorized as (C) for 75% of infected HCWs. No major issues with the doffing process were found. 98% of the HCWs performed a leak test upon use of N95 masks, 63% reported fitting problems. 75% of the infected staff spent more than 2 h in the COVID-19 area at a time. Four different kinds of N95 masks were used. Three of these masks were found not compatible for use and were disqualified by the IPC staff. The ward constituted of a clean area and a COVID area: after donning PPE in the clean area, COVID area is entered using an anteroom, HCWs use a different anteroom for doffing then back to the clean area. Only 2 air exchanges per hour in each room, with no negative pressure rooms. Corrective measures lead to control of the outbreak and included 2 main interventions: (1) using only N95 masks which were tested using standardized methods, and approved by the IPC team (2) improving ventilation system: increasing air exchanges and one-way-air-flow from clean to COVID area. Conclusion: Appropriate PPE and improvement of the ventilation and airflow in COVID-19 department lead to outbreak control. These actions improved staff safety and confidence among HCWs during patient care.

Disclosure of interest
None declared. Introduction: In view of the latest covid-19 pandemic condition worldwide, it affects greatly on paramedical colleagues daily practice especially on the suspected or not yet confirmed case transfer or evacuation. Unlike those server deadly infectious diseases case which goanna to have strict isolation devices, and most patients condition are ambulatory or transfer for isolation purposes only. Objectives: To develop a head to abdomen enclosed well covered jacket for transfer purposes in order to reduce the chance of droplets and aerosols spread whilst patient transfer to strict isolation areas. Thus, patient should feel comfortable and acceptable during the process. Methods: Soft plastic core shell on back and with hard transparent front was built; and loosely tight seal on all sides via elastic wrapper. Besides, airs will be sucked through HEPA filter to eliminate any infectious droplets or aerosols leak out to open air and infect those paramedical colleagues who simply escort the case to major Hospitals or strict isolation facility.
Results: Laboratory test conducted for its antimicrobial activities against Pseudomonas Aeruginosa (ATCC No. 9027), Enterobacteriaceae (Escherichia coli ATCC No. 8739); Staphylococcus aureus (ATCC No. 6538P); ISO 22196: 2011 Measurement of antibacterial activity on plastics and other non-porous surfaces methodology adopted and result indicated with its compliance of the antimicrobial activities. Trial run successfully with the simulated patient transfer and the safety standards as HEPA filter efficiency minimal standard well-kept in order to maintain the air seal internal environment via HEPA suctioning. In order to maintain the front shape for easy observation and comfort, it remodeled into hard shell. Jacket shell is used environmental friendly materials for disposable, combustible as of non-polyvinylchloride material.  Introduction: Several epidemic events caused by multi-resistant microorganisms following endoscopic procedures were reported despite strict adherence to endoscope reprocessing guidelines. Objectives: A nationwide cross-sectional study was conducted by the members of the scientific societies SITI (GISIO working group) and SIMPIOS to verify the effectiveness of reprocessing procedures of duodenoscopes. Methods: An ad hoc questionnaire was designed for collecting data about the management and activity of the endoscopy centers and those related to devices and their reprocessing and storage. Microbiological surveillance was conducted according to the "Duodenoscope Surveillance Sampling and Culturing Protocol", CDC 2018. Statistical analysis was carried out by the STATA SE14TM software (StataCorp LP-USA) and relationships between data were examined by the Spearman's rank correlation test. Results: Fifteen centers uniformly distributed in Italy participated, with a total of 144 post-reprocessing microbiological samplings performed on 55 duodenoscopes, of which 39.6% were found to be non-compliant, with high-concern (HC) and low-concern (LC) microorganisms detected in 29.2% and 10.4% of samples, respectively. The most frequently detected HC microorganisms were Pseudomonas aeruginosa (16%), followed by Escherichia coli (16%) and Klebsiella pneumoniae (14%). The mean value of microbial load relative to HC microorganisms was 441 ± 1196 (CFU/duodenoscope), with minimum and maximum values of 1 and 5400 CFU/duodenoscope. A statistically significant correlation was found between the microbial load of HC microorganisms and the storage time in non-ISO 16442 compliant cabinets before sampling (rho = 0.6834, p < 0.05).

Conclusion:
The preliminary results of the multicenter study showed a significant percentage (39.6%) of non-conformities in the microbiological investigation, incurred mainly due to HC microorganisms, and, consequently, a potential risk of cross-transmission associated with the use of duodenoscopes. Introduction: The detection of SARS-CoV-2 on inanimate surfaces lead to an increasing demand for environmental sampling solutions to detect microbiological contaminations. Monitoring of surface contamination was used to monitor potential implication for viral transmission and possible mitigation strategies using effective cleaning and disinfection procedures and precaution. Objectives: The purpose of this study was to evaluate viral RNA sample stability using Copan SRK ® , an environmental sampling kit consisting of a pre-moistened swab for sample collection and a reagent able to neutralize antimicrobial agents, for the detection of RNA Influenza A virus with molecular biology techniques. Methods: For the study, 100 μL of Influenza A virus (H3N2) (ATCC ® VR-1679 ™ ) viral suspension, chosed as representative of a ssRNA virus, were inoculated in 1 mL SRK tubes. To simulate product performances upon shipment, some SRK tubes were previously stressed performing a thermal shock. Additional 100 μL were inoculated in Copan UTM as reference. Viral nucleic acids were extracted with the ThermoFisher PrepSEQ ™ Residual DNA kit and the Applied Biosystem Automate express at different timepoints from inoculation. Samples were extracted at T0, after 2 and 7 days of storage at 2-8 °C and room temperature. Nucleic acids were stored at −20 °C until test (all samples were subjected to a freeze/thaw cycle before test). Detection of viral RNA was performed with the RIDA ® GENE real time PCR Flu/RSV. Additionally, to assess long term preservation, samples were extracted after 28 days at both 2-8 °C and RT starting from Copan SRK tubes. Results: Data obtained (Table 1) showed that Copan SRK is suitable to preserve inoculated samples and viral RNA from Influenza A virus can be detected up to 7 days from collection when stored at 2-8 °C and RT and up to 28 days at 2-8 °C, with a difference in Ct from T0 lower than 1 Ct (Fig. 1). As reference, samples stored at RT up to 7 days in UTM showed the same variation in Ct values from T0. No significant differences were observed when variation in Ct values is compared between SRK with and without the stress procedure. manual hygiene following the 6-step method. Manual hygiene monitoring can be carried out using different methods-direct monitoring, video filming, calculating the consumption of hand disinfectant etc. Objectives: The gold standard is a direct observation of health care worker hand hygiene, but due to pandemic situation, also infection control staff shortage, the new technologies should be implemented in health care for, Methods: Special data devices were installed in hospital clinics, to record all operations carried out at the sink, including hand-washing movements. For the video recording the IP cameras "POE-100CAMv2 used. Created 8 identical places near sinks (in the procedure rooms, ICU rooms) for obtaining video files near the sink for 24 h period/7 days a week. A special computer program was used to annotate video files. The developed program allows to encode each video frame with a special code, corresponded to a specific hand-washing movement recommended by the World Health Organization. The video files obtained at the hospital were recorded in principle-the recording is made and stored if a movement of at least 15 s is detected at the hand-washing site. All data manually reviewed and only hand washing episodes included in the study. Results: In general, cameras were placed in 8 locations and 14,886 video files were obtained, of which 2120 video was identified as valid for the study (i.e. the video content was hand washing). The rest of the video contained non-manual washing activities such as dishwashing. Medium hand washing time is 13 s. Compliance rate for all hand washing steps is 0.8%. Only 6 video files shows hand washing time more than 40 s. Figure / Image:

Conclusion:
Hand washing compliance is too low, but in the future the Influence of hand washing quality feedback on users behavior using a quality assessment tool based on neural networks/ simulated neural networks will be monitored on next study.

Disclosure of interest
None declared. Introduction: The adverse effects of coronavirus infection on pregnant women and their infants are not apparent. The best strategies to deal with this disease is avoiding the infection and preventing its transmission. Objectives: the present study aimed to investigate the relationship between demographic factors and levels of self-care against coronavirus in pregnant women referred to maternity wards of Kerman, Iran. Methods: The present descriptive study was conducted on 200 pregnant women who referred to maternity wards in Kerman, Iran in 2020 and met the inclusion criteria. The required information was collected using demographic questionnaires and a self-care checklist.

Results:
The mean age of the participants was 28.89 ± 7.07. Iranian and Afghan citizens comprised 82% and 18% of the participants, respectively. The highest level of self-care measures against coronavirus in pregnant women was attributed to the use of face masks (74%), and the lowest was warning the personnel to wear masks (28%). There was a statistically significant relationship between the nationality of the participants and warning the personnel to wear face masks (P = 0.02), the pregnant mothers' attention to wearing a scarf or cap during labor with the mothers' occupation (P = 0.006), having a sick spouse (P = 0.039), and having a sick child (P = 0.043), and between the patients' husbands' job and the patients' demand for a private room (P = 0.013).

Conclusion:
The results indicated that most pregnant women in the present study were active in self-care against coronavirus. Using face masks was more widely followed than other self-care measures; moreover, there was a relationship between personal characteristics and self-care levels.

Disclosure of interest
None declared.

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