Knowledge and perception on antimicrobial resistance and antibiotics prescribing attitude among physicians and nurses in Lambaréné region, Gabon: a call for setting-up an antimicrobial stewardship program

Background Africa is challenged by the emergence of antimicrobial resistance (AMR). In order to improve patient management and to optimise approaches to curb the spread of antimicrobial resistance, we examined knowledge and perceptions of AMR and antibiotics prescription practices of HCW (healthcare workers) in Lambaréné, Gabon. Methods We conducted a self-administered, questionnaire-based survey in HCW at the regional referral hospital, a medical research centre, and peripheral health care facilities. The proportions of correct responses to questions were determined and compared between physicians and nurses using Fisher’s Exact test. Results A total of 47 HCW took part in the survey. Of those, 64% (30/47) recognised antibiotic resistance as a major public health issue in Gabon, but only 14/47 (30%) recognised it as a problem in their health facility. Of note, 37/47 (79%) recognised excessive use of antibiotics without microbiological confirmation in case of infection, and buying antibiotics without a prescription, as possible cause of antimicrobial resistance. Some HCW (28%; 13/47) reported having prescribed antibiotics because the patients asked for them; and a total of 15/47 (32%) responded that antibiotics could help patients recover faster when added to malaria treatment. Compared to nurses, most of the physicians recognised that excessive use of antibiotics without microbiological confirmation of infection could contribute to AMR spread (18/19 (95%) vs 19/28 (68%); p = 0.028). Conclusion Most HCW recognised AMR as public health issue. However, a quarter of the participants did not know about the causes fostering the emergence of antimicrobial resistance. There is a need to perform regular HCW training in antimicrobial prescription, and to set up an antimicrobial stewardship program. Supplementary Information The online version contains supplementary material available at 10.1186/s13756-022-01079-x.

to proper microbiology services and other essential diagnostic tools increases the AMR burden [4,5].
The reduction of antimicrobial resistance requires rational use of antimicrobials, changes in prescription habits of healthcare workers (HCW), regulation of overthe-counter availability of antibiotics, improvements on hand hygiene, infection prevention and control [6]. Previous studies from Lambaréné and Libreville referral hospitals suggest a worrisome spread of antibiotic resistance in Gabon [7,8]. In samples from a referral hospital in Libreville, half of the isolated Klebsiella spp. were gentamicin-resistant; 30%, were resistant to tazobactam, and 18-79% were resistant to commonly-used cephalosporin antibiotics (ceftazidime, cefotaxime, cefoxitin, cefuroxime, and cefalotin).There was a high fluoroquinolone resistance rate, ranging from 18% ciprofloxacin to 54% nalidixic acid resistance [8]. From Lambaréné, high resistance rates against routinely used antibiotics of gram-negative bacteria isolated in the Albert Schweitzer Hospital were reported already a decade ago [7].
Antimicrobial therapy is an essential part of infectious disease management, with a focus on time-dependent recognition, as any delay to first-dose antibiotic administration can be associated with increasing mortality [9]. Therefore, HCW need to govern appropriate knowledge, attitudes and practices towards antimicrobial prescription. Information on physicians' and nurses' knowledge and awareness on AMR will permit the development of effective interventions and containment of AMR. Surveys have been conducted to assess physicians' knowledge and beliefs about antimicrobial use and resistance in developed countries [10,11]; however, data on sub-Saharan African's HCW awareness on AMR and antibiotic prescribing attitude such as Gabon is scarce. To date, there are no co-ordinated antimicrobial stewardship activities in Gabon. The routine and clinical data on microbiological resistance from the Lambaréné referral hospital show increasing AMR rates [7,12]. We hypothesised that there is a gap in knowledge of HCW about AMR. To our knowledge, this is the first study undertaken to assess the knowledge and awareness about antimicrobial resistance among physicians and nurses in Lambaréné, Gabon. The findings from this study would be useful in implementing preventive and control interventions on AMR at regional and national levels.

Study design and setting
We conducted a cross-sectional survey among HCW at a Lambaréné referral hospital (Albert Schweitzer Hospital; HAS), the Centre de Recherches Médicales de Lambaréné (CERMEL), and peripheral first-level health facilities (n = 6) in Moyen-Ogooué province of Gabon; from February to June 2020. HAS is one of the regional referral hospitals, and the peripheral health facilities are primary care level facilities. CERMEL conducts infectious diseases research and manages infectious disease outpatients in collaboration with HAS [13]. Although the regional hospital has a microbiology laboratory, antimicrobials are often prescribed presumptively. Health facilities included in our study fully represent the primary and intermediate levels healthcare services available in Lambaréné.

Survey instrument
We developed the questionnaire adapting content from previously published articles on the knowledge in HCW on AMR [11,14]. The questionnaire collected information on HCW's knowledge about antimicrobial resistance, attitudes about antibiotic prescribing, their perception of the importance of the problem of antimicrobial resistance, and their opinion about potential interventions designed to improve antibiotic use. The questionnaire was submitted in a pilot test to ten HCW to check comprehension and clarity of the questions (Additional file 1). The reliability of the questionnaire was tested, and the final questionnaire's Cronbach's alpha coefficient was 0.78.

Data collection
The researchers went to the study site to inform, explain, and discuss the study with potential participants. Participants were approached individually, and invited to undertake the survey after signing the written consent form. All HCW who were antimicrobial prescribers were invited to participate in the study. The questionnaire was self-administered. To maintain the anonymity and to make sure that researchers were unable to accidentally identify the participants, we delegated one participant from each department (not having been part of the research team) to collect all questionnaires, and to convey them to the research team. The phone number of the research team was provided to the participants in case of additional clarification having been required regarding answers provided.

Statistics
Study data were collected and entered by two independent data entry clerks into the Research Electronic Data Capture (RedCap) system [15], and any discrepancies were checked and corrected by a third individual. Percentages were calculated for categorical data. We used Fisher's exact test to compare finding between nurses and physicians. A two-sided p value of < 0.05 was considered statistically significant. The analysis was performed using R studio version 4.0.2 (Integrated Development Environment for R, Boston) [16].

Sample size
Due to the limited human resources of the health facilities of the Lambaréné region, all 53 individuals found to be eligible were invited to participate. We included all consenting HCW amongst those who were eligible (prescribers of antibiotics) and available.

Participant characteristics
Of the 53 eligible health workers, 47 subjects accepted the invitation to participate and all of them filled the questionnaire.

Antimicrobial resistance spreading awareness and knowledge
More than half (64%; 30/47) of the prescribers responded that antimicrobial resistance is a problem in Gabon, while 30% (14/47) responded that it is a problem in their health facilities. A total of 70% (33/47) of health workers responded that there is uncoordinated antimicrobial prescription in their health facility; and 81% (38/47) responded that self-medication of antimicrobials could contribute to antimicrobial resistance. Educational level (p = 0.89), the type of health facility (p = 0.45), the professional category (p = 0.38), and the length of service (p = 0.15) were not associated with a better awareness of AMR. The overall knowledge about possible causes of antimicrobial resistance spread was limited. The attitudes most frequently selected by HCW to be associated with AMR spreading were 'excessive use of antibiotics without microbiological confirmation of infection' (37/47, 79%) and 'buying antibiotics without a prescription' (37/47, 79%). In general, the proportion of appropriate responses to the questions related to possible cause of AMR spread was higher among physicians than among nurses (Table 1). However, the difference was not statistically significant, except for the questions which asked whether the 'excessive use of antibiotics without microbiological confirmation of infection could contribute to the spreading of AMR' (94.7% vs. 67.9%, p = 0.028; Table 1).

Antimicrobial prescribing attitude and practice
A total of 16/47 (35%) of participants across all professional groups prescribed at least three different groups of antibiotics during the week prior to the survey; 28% (13/47) reported to have prescribed antibiotics because the patients asked for them. The majority (55%; 26/47) of prescribers did not receive a recent training in antimicrobial prescription during the last year. Most of the HCW (85%, 40/47) referred to their own experience to choose the antibiotic to be prescribed to the patient; 21% (10/47) requested microbiological culture and antimicrobial susceptibility testing before prescribing antibiotics (Fig. 1). A total of 13/28 (46%) nurses versus 2/19 (10.5%) physicians (p = 0.023), reported that antibiotics, when added to malaria treatment, help the patients recover more quicker; 19/19 (100%) of physicians vs. 20/28(71%) nurses (p = 0.02) reported that antibiotics are always required for treating diarrhoea ( Table 2).

Ratings of the helpfulness of potential interventions to improve antibiotic prescription
All of (47/47,100%) respondents strongly agreed that it would be very useful to perform regular training on antimicrobial prescriptions; and to have guidelines for antibiotic prescription; as well as that the vast majority agreed to determine the local antimicrobial resistance profile to guide empirical prescription 90% (42/47) ( Table 3). Across all professional levels, HCW (91%; 43/47) agreed that setting up action to control the prescription of antimicrobials would be very useful in their health facility, but not to restrict the prescription of all antibiotics.

Discussion
As per our knowledge, this is the first study assessing AMR knowledge and antibiotics prescription practice among HCW in Gabon. Similar to other studies, the prescribers responded that antimicrobial resistance is a global and national public health challenge [17][18][19]. However, few participants recognised AMR as a problem in their health facilities. These findings are consistent with studies conducted in Ethiopia [20], Congo [21] and Sudan [22]. In contrast, studies from Peru [23] and Brazil [24] reported that the majority of prescribers perceived AMR as a problem in their health facilities. Study participants knew little about practices that contribute to the spreading of antimicrobial resistance. That could be explained by a lack of regular training on antimicrobial drugs prescription and AMR development. The lack of training contributes to antibiotic irrational prescription, and it is associated with AMR spread [1,17,25]. Fifteen HCW (32%) responded that antibiotics help patients recover faster when added to malaria treatment; a statement which should not be easily dismissed as incorrect, as a fair proportion of children (around 6.5%) with severe falciparum malaria in sub-Saharan Africa were reported to be concomitantly bacteraemic [20]. Rational antibiotics prescription is the main strategy to prevent AMR [6]. This is achieved by changing the prescribers' attitude and practice. The results of this study suggest that misperceptions about antibiotic use prevailed, which may cause unnecessary prescription, as reported previously [26,27]. According to this study, as in the study presented here, the antibiotics prescribers' decision-making was sometimes influenced by patient requests (28%). Rodrigues et al. [28] and Asante et al. [14] reported similar findings. Patients' influence on prescription of specific antibiotics is also a factor for excessive antibiotics prescription. Prescribers should explain the rationale of antibiotic prescriptions to the patients. Patients are sometimes not aware of the consequences of poor antibiotics prescription practices and AMR development [29,30], with some of them pushing hard for prescription of antibiotics. Effective physician-patient communication and patient empowerment reduce antibiotics prescriptions [31]. Moreover, our findings stress the need to restrict the over-the-counter sale of certain antibiotics without a physician's prescription.
Regarding potential interventions to combat AMR based on our findings, most favourite measures include: (1) to establish a national AMR surveillance program; and (2) to avail clinical microbiology laboratory capacity and local guidelines for rational use of antibiotics (Table 3); (3) to provide education on antimicrobial stewardship for health professional interventions. Using similar questionnaires, studies conducted in health workers from Saudi Arabia [25], and Ghana [14], and others focusing on junior doctors from Ethiopia [32], France and Scotland [11] suggested similar interventions. Only 30% (14/47) of the interviewed health workers responded that poor hand hygiene can contribute to AMR spread. This finding stresses the need of regular infection prevention and control training of health workers. A large percentage of healthcare-associated infections are preventable by improving hand hygiene practices [33]. Hand hygiene is an efficient infection prevention and control strategy in the fight against AMR transmission [34]. In fact, it is the key component of effective infection prevention and control programs recommended by WHO [35]. As expected, most physicians had a good attitude concerning the prescription of antimicrobials, compared to nurses. However, all of the physicians reported that antibiotics are always required for diarrhoea. This is an unexpected finding, which can be possibly explained by the fact that the most-frequently isolated pathogens in diarrhoea of children under five years in Lambaréné and surroundings were entero-invasive Escherichia coli/Shigella spp. (30.2%), enterotoxigenic Escherichia coli (24.6%), followed by G. lamblia (13.7%), Cryptosporidium spp. (12.9%), and rotavirus (9.5%) [36].
The strength of this study is that we included all level of health facilities in the Lambaréné region. Furthermore, this study provides valuable information on AMR and antimicrobial prescription, that could help to design appropriate local antimicrobial stewardship measures. Gabon has no formal AMR surveillance system as recommended by WHO [37]. It is important that a national or regional AMR surveillance be established to regularly update and disseminate AMR data to all HCW.
As with most surveys, our study does have certain limitations; for example, there is a possibility that respondents gave what was perceived as socially most acceptable answers. Furthermore, the sample size in our study is low compared with other studies conducted earlier in Africa reflecting the lack of HCW particularly outside the capitals in sub-Saharan Africa. We invited all HCW in the target health facilities to participate without restrictions. The small sample size of our study may limit the generalisability of our findings to national level, and the interpretation of our study results should be done with caution. There is a need to search for funding to conduct a national survey to further understand the issue of AMR in Gabon and to take appropriate actions to reduce its spread.

Conclusions
Prescribers in Lambaréné recognise the AMR as a public health challenge in health facilities and in Gabon. Prescribers strongly believed that antimicrobial stewardship should be implemented in their health facilities to reduce the spread of AMR. There is a need to set up regular (re) training programs in antimicrobial prescription across health facilities in Lambaréné, Gabon.    Availability of the opinion of a pharmacist 27 (57) 13 (28) 7 (15) Availability of the operational hygiene team 27 (57) 9 (19) 11 (23) Have access to computerized prescribing assistance 40 (85) 5 (11) 2 (4) Restrict the prescription of certain antibiotics (then requiring specialist advice) 43 (91) 1 (2) 3 (6) Restrict the prescription of all antibiotics 22 (47) 7 (15) 18 (38) Regularly assess the prescription of antibiotics in a department, return the information to prescribers and possibly implement actions to improve the prescription 44 (94) 0 (0) 3 (6)