Evaluation of a novel surface-coating formulation with time-extended antimicrobial activity for healthcare environment disinfection

Table 3 Conditions used for the laboratory phase experiment with OxiLast™ coating

Objective	CT (minutes)	Coating (days)	Tested strains	Tested surfaces	CFU inocula used
General efficacy testing	5, 15, 30	0, 1, 2, 3, 5, 7	P. aeruginosa ATCC 9027	64 cm² PVC; 3 tests 1 control	10⁷ for all CT 15 and 30 min, and CT 5 min at days 0 to 2. 10⁶ for CT 5 min on days 3, 5, and 7
			MRSA ATCC 33591	64 cm² PVC; 3 tests 1 control	10⁷ for all CT 30 min, and for CT 15 min at day 0. 10⁶ for CT 5 min at day 0 and all CT 15 and 30 min on days 1 to 7
			A. baumannii ATCC 19606	64 cm² PVC; 3 tests 1 control	10⁷ for all
			K. pneumoniae ATCC BAA-1705	64 cm² PVC; 3 tests 1 control	10⁷ for all CT 15 and 30 min, and CT 5 min on days 0 to 2. 10⁶ on CT 5 min on days 3 to 7
			VRE ATCC 51299	64 cm² PVC; 3 tests 1 control	10⁶ for all
Organic load	15, 30	0, 1, 2, 3, 5, 7	P. aeruginosa ATCC 9027	64 cm² PVC; 3 tests 1 control	10⁶ for all
Organic load	15, 30	0, 1, 2, 3, 5, 7	MRSA ATCC 33591	64 cm² PVC; 3 tests 1 control	10⁶ for all
Successive loading	15 + 15	0, 1, 2, 3, 5, 7	P. aeruginosa ATCC 9027	64 cm² PVC; 3 tests 1 control	10⁶ first inoculum and 10⁵ second inoculum
Successive loading	15 + 15	0, 1, 2, 3, 5, 7	MRSA ATCC 33591	64 cm² PVC; 3 tests 1 control	10⁶ first inoculum and 10⁵ second inoculum
Mechanical abrasion	30	0	P. aeruginosa ATCC 9027	64 cm² PVC; 3 tests 1 control	10⁶ for all
Mechanical abrasion	30	0	MRSA ATCC 33591	64 cm² PVC; 3 tests 1 control	10⁶ for all

ISSN: 2047-2994