Application of MBPs sprayed on surfaces in hospital environment is significantly more efficient method of elimination of MDRBSs compared to their usage by any other way. MBPs possessed greater efficiency on glass, metal, and plastic surfaces compared to textile and paper. Duration of MBP circulation was determined by time frame, MBP strain, and lytic activity of the MBP, but not by dose of MBP on the surface. The greatest effect was revealed against PA; even the single usage of MBP provided total elimination of PA from the hospital environment. The application of MBPs led to 15-fold decrease of incidence of Salmonella-caused infections, 4-fold reduction of incidence of Shigella-caused infections, and 2-fold decrease of incidence of Klebsiella- and MRSA-caused infections. Finally, CAMs did not influence the efficiency of MBPs, and no side effects were registered.