A case series design was applied. Study period: 28 May to 15 October, 2012. Study population: 166 nurses in main HCFs in Isiro, HCF A (n=47, 70 beds), HCF B (n=32, 70 beds), HCF C (n=57, 160 beds), HCF D (n=30, 53 beds). Case definition established suspect, probable and confirmed (laboratory confirmed by RT-PCR or serology) cases. Active early case detection and infection prevention and control was promoted in all HCFs; an isolation unit established in HCF C on August 10, 2012 suspect cases were referred. Laboratory tests were performed in all suspect cases and HCWs who expressed interest. To assess potential risk factors, the HCFs in the Isiro urban-rural health zone were assessed for IPC practices and hand hygiene facilities, and a structured questionnaire applied to nurses who had been tested for BE infection to assess contact with BE cases, service and HCF where the nurse was assigned. Two-tailed exact test was used when appropriate.