The survey was returned by 554 HCWs. The majority of participants were female(87.2%) and average age was 29.4(SD=6.15). In profession, participants were consisted of nurses(62.8%), technologists(17.5%), nursing assistants(14.6%) and physicians(4.2%). On the perception of practice, 65.7% of HCWs answered they had 10-30 occasions for hand hygiene per day and 44.6% of HCWs reported self-administered compliance by 50-80%. In usability, antimicrobial liquid soap(49.1%) was preferred to alcohol gel(31.6%). The most cleansed part was palm(62.3%) and uncleansed part was wrist(43.1%)and fingertip(29.8%). The reasons of non-compliance were “too busy”(63.5%), skin reaction(20.2%), lack of perception(19.9%), and resource shortage(17.5%). Many HCWs experienced skin irritation(52.7%). HCWs thought that “alcohol gel is convenient”(76.7%), “makes hands dry”(55.1%), “dry faster”(27.1%) and the antimicrobial liquid soap “makes hands dry”(51.6%), “foam insufficiently”(24.7%), “inconvenient”(14.1%).