A total of 1271 HH opportunities were identified during the observation period. Overall HH compliance was 33% (417/1271) of which 58.8% were appropriate. Compliance differed by role: nurses (49%), physicians (24%) and others (19 %) (p≤0.001). Healthcare workers (HCWs) and visitors were more likely to use soap and water (76.1%) compared to ABHR (23.9%) and no significant difference was detected among these groups (p=0.330). In regards to type of department, the use of ABHR was found to be strongly higher in Surgical wards (71.8%) compared to the rest of wards which this rate ranges from 11.1% in NICUs to 33.3% in emergency departments (p<0.001). The HH procedure was appropriate in 63.4% and 45.4% among those used soap and water and ABHR, respectively (p=0.002). The most commonly identified HH opportunities were after child contact (381), before child contact (376), after contact with child’s surroundings (358) and before aseptic procedure (95). Despite the fact that all HCWs use more often hand washing, a minor number of HH opportunities (61) were identified after contact with body fluids, the step of HH which demands this HH method.