Among 85 wards, 388 staff from 30 wards participated in this study. The hand rubbing time for all participants were more than the recommended 10-15 seconds with an average of 57±26.4 seconds. The hand rubbing time was not affected by age, gender, and professional categories. 45.2% have zero residuals. 74.7% had less than 3 residue points. The average residue point is 2±2.8 points. There is no correlations among hand rubbing time, participants’ preceded confidence, and the residue points. We found that participants who have damaged skin had more residues. The highest percentage of the residue points lie in the tips of the nails (38.6%, 340/880), followed by figure tips (17.4%). Follow-up survey showed the proportion of staff with correct HH technique increased from 76.6% in 2011 to 81.3% in 2012. The composite compliance rate increased as well (from 82.7% to 85.5%).