During the study period, 348 HCWs were surveyed. The surveyed HCWs were nurses(55.5%), physicians(4%), technicians(25.6%), and nurse assistants(14.7%). The mean age was 30.2(SD=6.6) and the majority of participants were female(76.4%). The mean score of hand hygiene knowledge was 14.25(SD=2.05), there were significantly differences in gender (female, t=-2.276, P=.023), clinical experience(above 5years, t=-2.463, P=.014), profession(nurse, F=9.337, P<.01) and no significantly differences in age, department. Otherwise the mean score of hand hygiene perception was 75.2(SD=11.83), there were significantly differences in age(above 31years, t=-3.224, P=.001), profession(nurse, F=4.1, P=.007), department(ICU, F=2.57, P=.038) and no significantly difference in gender, clinical experience. Having had a formal training in hand hygiene was significantly difference both of knowledge(t=5.50, P<.01) and perception(t=2.4, P=.017).