69 Acinetobacter strains were isolated from samples of 64 patients who were hospitalized in ICUs. Acinetobacter infections have became two times in five patients. 21 of patients were female, 43 of them were male and average age was 61,09±24,16 (2-91) years. Distributions of Acinetobacter infectious of ICUs were investigated and the most infectious (49,3% n:34) were occured at medical ICU followed by anesthesia recovery unit(20,3% n:14), burn unit (8,7% n:6), ICU of general surgery unit(7,2% n:5), ICU of neurology unit (5,8% n:4), respectively. The avarage of interval between hospitalization date and devolopment time of infectious was 27,78±40,02 (3-282) days and it varies according to the different ICUs Bloodstream infection was the most common infection, followed by urinary tract infection, pneumonia (n:10 14,4%), skin and soft tissue infections (n:8 11,6%), respectively. The most common species was A. baumanni (n:45 65,2%) and A. calcoaceticus (n:3 4,3%) was the second. When antimicrobial susceptibility patterns of isolated acinetobacter spp were investigated, susceptibility rates were determined as 100% to colistin, 27,2% to tigecycline, 25% to amikacin, 19,1% to gentamicin, 18,4% to cotrimoxazole, 5,1% to ciprofloxacin, 4,4% to piperacillin-tazobactam, 3,3% to aztreonam, 3,2% to cefotaxime, 3,1% to ceftazidime, 1,4% to imipenem and meropenem.