Three manual and three automatic procedures were evaluated with at least 3 dispensers per procedure. Most experiments were done with contaminated dispensers from healthcare facilities. In addition, some experiments were done with new dispensers which were freshly contaminated with contaminated solution from a clinical dispenser. After processing, the dispenser was refilled with a surface disinfectant based on benzalkonium chloride (Mikrobac forte, 0.5%), the new tissue role inserted and the filled dispenser left at room temperature. Disinfectant samples were taken after 7, 14, 21 and 28 days. The number of CFU per mL was determined by serial dilution using standard microbiological techniques.