The antibiotic susceptibilityshowed the KP producing novel KPC variant was resistant to most of the antibiotics, such as carbapenem (imipenem, ertapenem and meropenem), aztreonam, cephalosporin (cefazolin, cefotaxime and ceftazidime), but susceptible to amikacin and colistin. The whole genome sequencing has been done and resulted in 11 contigs needed to be assembled. The genetic environment surrounding novel blaKPC flanked by ISKpn8 and ISKpn6-like sequences is similar with pKP048. The sequences upstream of ISKpn8 in pKP78 were, with gene order TniA transposase, IS26 transposase and partial Tn3-resolvase different from Tn3-transposase and Tn3-resolvase in pKP048. The GST-recombinant proteins were expressed and the detection of enzymatic activity is undertaken.