Panelists representing a HCW demographic were studied over a two week period. Following a washout period, skin assessments and treatments were conducted at regular intervals. Three ABHR systems containing 70% alcohol (ethanol, isopropanol, or n-propanol), water, and humectant were used in addition to a control (water and humectant only). Panelist forearms received eight randomized regimens: three alcohol systems applied 20 times per day (standard frequency; SF); three alcohol systems applied 100 times per day (high frequency HF); an untreated skin control; and the water/ humectant system applied 100 times per day. Panelist’s forearms were also washed six times per day at scheduled intervals of a minimal HCW daily routine. Skin redness and dryness, skin hydration, and skin barrier were measured to assess each unique regimen. Analysis of variance was used to assess the individual and interactive effects of alcohol type and application rate, and to compare to the untreated and alcohol-free treatments.