- Poster presentation
- Open access
- Published:
Molecular characteristic of imipenem-resistant Pseudomonas aeruginosa isolated from unrinary tract infections in Southern Poland
Antimicrobial Resistance and Infection Control volume 4, Article number: P139 (2015)
Introduction
Carbapenem-resistant Pseudomonas aeruginosa (PAR) has become a serious health problem worldwide. It is essential to understand its epidemiology as it may help to control the antibiotic resistance.
Objectives
To analyze the molecular characteristics of carbapenem-resistant PAR in urinary tract infections in Southern Poland.
Methods
Antimicrobial susceptibility testing was performed. Metallo-beta-lactamases were detected. Multidrug-resistant (MDR) was non-susceptible to one antimicrobial in ≥3 antimicrobial classes. Extensively-drug resistant strain (XDR) was susceptible to ≤2 antimicrobial classes. MLST was performed (Curran et al ,2004).
Results
The median (Q1;Q3) age was 60 years (54;69), 33.3% were females. Among 183 urine samples contained P. aeruginosa, 21 imipenem-non-susceptible strains were included for further analysis. MIC50 for imipenem was 12.0 mg/l. Eighteen strains (86.0%) were resistant to meropenem (MIC50=8.0 mg/l). Sixteen strains (76.0%) were resistant to doripenem. Based on the EDTA-assay, 9 (42.8%) MBL-positive isolates were identified. VIM-2 was present in three isolates. No isolates with SPM nor IMP, SIM, GIM were detected. Three (14.2%) isolates were classified as MDR, 8 as XDR (38%). MDR/XDR strains were found more often among polimycrobial infections than monomicrobial (p=0.042, OR=0.093, 95% CI 0.0085-1.00). Eight XDR strains were designated to MLST typing scheme. Four strains belonged to ST235, two strains to ST 260. The remaining two strains belonged to ST654 o ST234, respectively.
Conclusion
This study indicated the emergence of MDR and XDR strains producing MBL. A high prevalence of imipenem-resistant strains and MBL is a critical problem and a therapeutic challenge for clinicians. Continuous surveillance is necessary to detect the presence of MBL-producing strains. No 2012/05/N/NZ7/00786.
Disclosure of interest
None declared.
Author information
Authors and Affiliations
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.
The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Pobiega, M., Maciag, J., Chmielarczyk, A. et al. Molecular characteristic of imipenem-resistant Pseudomonas aeruginosa isolated from unrinary tract infections in Southern Poland. Antimicrob Resist Infect Control 4 (Suppl 1), P139 (2015). https://doi.org/10.1186/2047-2994-4-S1-P139
Published:
DOI: https://doi.org/10.1186/2047-2994-4-S1-P139